Simple, rapid, and accurate detection of myoinositol (MI) concentration in blood is crucial in diagnosing polycystic ovary syndrome, neurological disorders, and cancer. A novel electrochemical detection (IED) method was established to quantify MI in human serum using a disposable unmodified screen-printed carbon electrode (SPCE) for the first time. MI was detected indirectly by the reaction product of myoinositol dehydrogenase (IDH) and cofactor β-nicotinamide adenine dinucleotide (NAD⁺). Good linear calibration curves were obtained at the concentration range from 5.0 μM to 500.0 μM (R² = 0.9981) with the lower limits of detection (LOD) and quantification (LOQ) of 1.0 μM and 2.5 μM, respectively. Recoveries were calculated at three spiked concentrations, and the values were between 90.3 and 106%, with relative standard deviation values of 3.2–6.2% for intraday precision and 7.1–9.0% for interday precision. The SPCE-electrochemical biosensor is simple, accurate, and without modification, showing great potential for point-of-care testing (POCT) of serum MI in clinical samples.

中文翻译：

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使用未经修饰的丝网印刷碳电极通过电化学检测定量血清中的肌醇

简单、快速和准确地检测血液中的肌醇 (MI) 浓度对于诊断多囊卵巢综合征、神经系统疾病和癌症至关重要。首次使用一次性未改性丝网印刷碳电极 (SPCE) 建立了一种新的电化学检测 (IED) 方法来量化人血清中的 MI。MI通过肌醇脱氢酶(IDH)和辅因子β-烟酰胺腺嘌呤二核苷酸(NAD ⁺ )的反应产物间接检测。在 5.0 μM 至 500.0  μM ( R ² = 0.9981 )的浓度范围内获得良好的线性校准曲线  ，检测下限 (LOD) 和定量 (LOQ) 为 1.0  μM 和 2.5  μ M，分别。在三个加标浓度下计算回收率，数值介于 90.3 和 106% 之间，日内精度的相对标准偏差值为 3.2-6.2%，日间精度的相对标准偏差值为 7.1-9.0%。SPCE-电化学生物传感器简单、准确且无需修改，在临床样本中对血清 MI 的即时检测 (POCT) 显示出巨大的潜力。