Zhachong Shisanwei Pills, composed of 13 Chinese medicinal materials, are used for treating the diseases such as hemiplegia, pain of muscles and bones, rheumatism, and joint pain. The chemical composition and pharmacodynamics of Zhachong Shisanwei Pills have not been reported. Ultra-performance liquid chromatography/quadrupole-time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS/MS) was employed to quickly identify the chemical components of Zhachong Shisanwei Pills, which was performed with Shim-pack GIST C_(18) column(4.6 mm×150 mm, 5 μm). The gradient elution was conducted with methanol-0.05% acetic acid as the mobile phase. Electrospray ionization mass spectrometry(ESI-MS) was carried out in both positive and negative ion modes. The compounds were identidied based on accurate relative molecular weight, fragment ion species, and the MS data of reference substances and in literature. In conclusion, a total of 98 compounds were identified, including 19 organic acids, 36 flavonoids, 13 volatile oils, 8 tannins, 5 2-(2-phenylethyl)chromones, 5 amino acids, 3 sesquiterpenoids, 3 alkaloids, and 2 other compounds. This study characte-rized the chemical components of Zhachong Shisanwei Pills rapidly for the first time, laying a foundation for further research on the pharmacodynamic material basis and quality evaluation.

中文翻译：

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UPLC-Q-TOF-MS/MS对扎虫十三味丸化学成分的鉴定[J].

扎冲十三味丸由13种中药组成，用于治疗偏瘫、筋骨痛、风湿、关节痛等疾病。乍虫十三味丸的化学成分和药效学尚未见报道。Shim-pack GIST C_( 18）柱（4.6 mm×150 mm，5 μm）。以甲醇-0.05% 乙酸为流动相进行梯度洗脱。电喷雾电离质谱（ESI-MS）在正离子和负离子模式下进行。根据准确的相对分子量、碎片离子种类、以及参考物质和文献中的MS数据。总之，共鉴定出98种化合物，其中有机酸19种、黄酮类36种、挥发油13种、单宁8种、2-(2-苯乙基)色酮5种、氨基酸5种、倍半萜类3种、生物碱3种、其他化合物2种. 本研究首次快速表征了扎虫十三味丸的化学成分，为进一步研究药效物质基础和质量评价奠定了基础。