Background

Previous studies have demonstrated that pre-exercise suppresses anxiety-like behavior, but the effects of different exercise times on vascular dementia induced anxiety-like behavior have not been well investigated.

Objective

The present study aims to investigate the underlying neurochemical mechanism of different pre-vascular-dementia exercise times on 5-HT and anxiety-like behavior in rats with vascular dementia.

Methods

32 Sprague-Dawley (SD) rats were randomly divided into 4 groups: sham group (S group, n = 8), vascular dementia group (VD group, n = 8), 1-week physical exercise and vascular dementia group (1WVD group, n = 8), and 4 weeks physical exercise and vascular dementia group (4WVD group, n = 8). 1 week and 4 weeks of voluntary wheel running were used as pre-exercise training. The vascular dementia model was established by bilateral common carotid arteries occlusion (BCCAo) for 1 week. But bilateral common carotid arteries were not ligated in the sham group. The level of hippocampal 5-HT was detected with in vivo microdialysis coupled with high-performance liquid chromatography (MD-HPLC). Elevated plus maze (EPM), open field (OF), and light/dark box test were used to test anxiety-like behavior.

Results

Compared with the C group, the hippocampal 5-HT was significantly decreased in the VD group after 1 week of ligated operation. The hippocampal 5-HT levels in 1WVD and 4WVD groups were substantially higher than the level in the VD group. The hippocampal 5-HT level has no significant difference among C, 1WVD, and 4WVD. Behavioral data suggested that the rats in the VD group developed obvious anxiety-like behavior after 1 week of ligation surgery. Still, the rats in 1WVD and 4WVD groups did not show significant anxiety-like behavior.

Conclusion

Both 1 week and 4 weeks of voluntary running wheel exercise can inhibit the anxiety-like behavior in rats with vascular dementia by upregulating 5-HT levels in the hippocampus in the VD model.

中文翻译：

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不同运动时间对血管性痴呆大鼠5-HT及焦虑样行为的影响

 背景

先前的研究表明，运动前可以抑制焦虑样行为，但不同运动时间对血管性痴呆诱发的焦虑样行为的影响尚未得到很好的研究。

 客观的

本研究旨在探讨血管性痴呆前不同运动时间对血管性痴呆大鼠5-HT和焦虑样行为的潜在神经化学机制。

 方法

32只SD大鼠随机分为4组：假手术组（S组，n=8）、血管性痴呆组（VD组，n=8）、1周体育锻炼+血管性痴呆组（1WVD组） ，n = 8），以及4周体育锻炼和血管性痴呆组（4WVD组，n = 8）。 1周和4周的自愿轮跑作为运动前训练。采用双侧颈总动脉闭塞(BCCAo)1周建立血管性痴呆模型。但假手术组不结扎双侧颈总动脉。采用体内微透析结合高效液相色谱法 (MD-HPLC) 检测海马 5-HT 水平。高架十字迷宫（EPM）、开放视野（OF）和明/暗盒测试用于测试焦虑样行为。

 结果

与C组相比，结扎手术1周后VD组海马5-HT明显降低。 1WVD和4WVD组的海马5-HT水平显着高于VD组。 C、1WVD、4WVD组海马5-HT水平无显着差异。行为数据表明，VD组大鼠在结扎手术1周后出现明显的焦虑样行为。尽管如此，1WVD 和 4WVD 组的大鼠并未表现出明显的焦虑样行为。

 结论

1周和4周的自愿跑轮运动均可通过上调VD模型海马5-HT水平来抑制血管性痴呆大鼠的焦虑样行为。