Abstract

Microbiologically influenced corrosion and biofouling of steels depend on the adsorption of a conditioning film and subsequent attachment of bacteria. Extracellular deoxyribonucleic acid (eDNA) and amino acids are biologically critical nutrient sources and are ubiquitous in marine environments. However, little is known about their role as conditioning film molecules in early biofilm formation on metallic surfaces. The present study evaluated the capacity for eDNA and amino acids to form a conditioning film on carbon steel (CS), and subsequently, the influence of these conditioning films on bacterial attachment using a marine bacterial strain. Conditioning films of eDNA or amino acids were formed on CS through physical adsorption. Biochemical and microscopic analysis of eDNA conditioning, amino acid conditioning and control CS surfaces demonstrated that organic conditioning surfaces promoted bacterial attachment. The results highlight the importance of conditioning the surface in initial bacterial attachment to steel.

摘要

微生物影响钢的腐蚀和生物污垢取决于调节膜的吸附和随后的细菌附着。细胞外脱氧核糖核酸 (eDNA) 和氨基酸是生物学上至关重要的营养来源，在海洋环境中无处不在。然而，关于它们作为调节膜分子在金属表面早期生物膜形成中的作用知之甚少。本研究评估了 eDNA 和氨基酸在碳钢 (CS) 上形成调理膜的能力，以及随后这些调理膜对使用海洋细菌菌株的细菌附着的影响。通过物理吸附在 CS 上形成 eDNA 或氨基酸的调理膜。eDNA调节的生化和微观分析，氨基酸调理和对照 CS 表面表明有机调理表面促进细菌附着。结果突出了在最初的细菌附着于钢时调节表面的重要性。