In acidic soil, potassium (K) deficiency is a main limiting factor that restricts the growth of fruit trees. Biochar has been used as a soil amendment to increase pH and nutrients. However, studies on the effect of biochar on the bacterial community and soil properties in potted experiments are limited. Therefore, we used peanut shell biochar to investigate the effect of biochar on the structure and function of bacterial communities by high-throughput sequencing, soil enzymes (urease, dehydrogenase, and extracellular enzymes), and gene function prediction in response to K deficiency in acidic soil. The results indicated that soil pH, enzyme activity, and available nutrients were improved by biochar. In particular, biochar still had a beneficial effect on improving soil and plant K levels after thirty months of application. In addition, biochar promoted the growth of K-dissolving bacteria and relative abundance of bacteria (p_actinomycetes, p_chloroflexi, p_proteobacteria, p_acidobacteriota, and p_firmicutes). Based on the above results, we assumed that biochar application can effectively improve soil K levels and bacterial functions. Such as: K-dissolving, soil organic matter decomposition and the conversion of nutrients. Our findings encourage and support the use of biochar as an amendment in acidic soil.

在酸性土壤中，钾（K）缺乏是限制果树生长的主要限制因素。Biochar 已被用作土壤改良剂以增加 pH 值和养分。然而，在盆栽实验中，关于生物炭对细菌群落和土壤性质影响的研究有限。因此，我们使用花生壳生物炭，通过高通量测序、土壤酶（脲酶、脱氢酶和细胞外酶）和响应酸性缺钾的基因功能预测，研究生物炭对细菌群落结构和功能的影响。土壤。结果表明，生物炭提高了土壤pH值、酶活性和速效养分。特别是，生物炭在施用 30 个月后仍然对改善土壤和植物 K 水平具有有益作用。此外，p_actinomycetes、p_chloroflexi、p_proteobacteria、p_acidobacteriota和p_firmicutes）。基于上述结果，我们假设生物炭的应用可以有效改善土壤钾含量和细菌功能。如：钾的溶解、土壤有机质的分解和养分的转化。我们的研究结果鼓励并支持使用生物炭作为酸性土壤的改良剂。