The biocatalytic activity of electrode-immobilized luciferase followed by bioluminescence emitted from the electrode surface was reversibly tuned and switched by applying electrochemical signals. When a reductive potential (−0.9 V vs. Ag/AgCl) was applied, O₂ was consumed at the electrode resulting in its depletion in a thin film near the electrode surface. This resulted in the inhibition of the immobilized luciferase which needs O₂ for the biocatalytic reaction. Releasing the potential resulted in diffusional equilibration of the O₂ local concentration with the bulk solution, then reactivating luciferase. Reversible inhibition-activation of luciferase was obtained upon cyclic application and releasing of the potential, respectively.

通过施加电化学信号可逆地调节和切换电极固定荧光素酶的生物催化活性，然后从电极表面发出生物发光。当施加还原电位（-0.9 V vs. Ag/AgCl）时，O ₂在电极处被消耗，导致其在电极表面附近的薄膜中耗尽。这导致了生物催化反应需要O _{2的固定化荧光素酶的抑制。}释放电位导致 O ₂局部浓度与本体溶液的扩散平衡，然后重新激活荧光素酶。在循环施加和释放电位时分别获得了荧光素酶的可逆抑制-激活。