Cryo–electron microscopy (cryo-EM) continues its remarkable growth as a method for visualizing biological objects, which has been driven by advances across the entire pipeline. Developments in both single-particle analysis and in situ tomography have enabled more structures to be imaged and determined to better resolutions, at faster speeds, and with more scientists having improved access. This review highlights recent advances at each stageof the cryo-EM pipeline and provides examples of how these techniques have been used to investigate real-world problems, including antibody development against the SARS-CoV-2 spike during the recent COVID-19 pandemic.

中文翻译：

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更好、更快、更便宜：冷冻电子显微镜的最新进展


冷冻电子显微镜（cryo-EM）作为一种可视化生物物体的方法，在整个管道的进步的推动下，继续其显着的发展。单粒子分析和原位断层扫描的发展使得更多的结构能够以更好的分辨率、更快的速度进行成像和确定，并且更多的科学家能够更好地获取这些结构。本综述重点介绍了冷冻电镜管道各个阶段的最新进展，并提供了如何使用这些技术来研究现实世界问题的示例，包括在最近的 COVID-19 大流行期间针对 SARS-CoV-2 峰值的抗体开发。