Sample preparation is considered as the bottleneck step in bioanalysis because each biological matrix has its own unique challenges and complexity. Competent sample preparation to extract the desired analytes and remove redundant components is a crucial step in each bioanalytical approach. The matrix effect is a key hurdle in bioanalytical sample preparation, which has gained extensive consideration. Novel sample preparation techniques have advantages over classical techniques in terms of accuracy, automation, ease of sample preparation, storage, and shipment and have become increasingly popular over the past decade. Our objective is to provide a broad outline of current developments in various bioanalytical sample preparation techniques in chromatographic and spectroscopic examinations. In addition, how these techniques have gained considerable attention over the past decade in bioanalytical research is mentioned with preferred examples. Modern trends in bioanalytical sample preparation techniques, including sorbent-based microextraction techniques, are primarily emphasized.

样品制备被认为是生物分析的瓶颈步骤，因为每种生物基质都有其独特的挑战和复杂性。有效的样品制备以提取所需的分析物并去除多余的成分是每种生物分析方法中的关键步骤。基质效应是生物分析样品制备中的一个关键障碍，已得到广泛考虑。新型样品制备技术在准确性、自动化、样品制备、存储和运输的便利性方面优于经典技术，并且在过去十年中变得越来越流行。我们的目标是概述色谱和光谱检查中各种生物分析样品制备技术的当前发展。此外，在过去十年中，这些技术如何在生物分析研究中获得了相当大的关注，并举了一些优选的例子。主要强调了生物分析样品制备技术的现代趋势，包括基于吸附剂的微萃取技术。