Electrical stimulation has been demonstrated to have beneficial effects in skin tissue repair. However, most electrical stimulations are applied with percutaneous electrode, which is prone to causing serious trauma. Using non-contact electrical stimulation (NCES) is expected to reduce the potential risk. In this study, NCES was expediently exerted by a self-designed practical device. Electrode plates of 10-cm spacing with appropriate side lengths of 21 and 30 cm were selected by EF distribution analysis for applying NCES to cells and mice, respectively, and the real EF strengths were measured. The change of loading voltage which had no effect on the regular pattern of EF distribution could be used as a single factor to explore the effect of NCES on wound healing. It was subsequently demonstrated that 53 mV mm⁻¹ NCES facilitated the migration and proliferation of HaCaT cells and HDFs in vitro, and the M2-type polarization of macrophages. Moreover, 54 and 84 mV mm⁻¹ NCESs accelerated the wound healing rate of model mice from the perspective of reducing scarring, enhancing collagen synthesis and increasing angiogenesis in vivo. The promoting role of NCES in wound healing showed the potential to initiate new possibilities for the clinical treatment of skin tissue injuries.

已证明电刺激在皮肤组织修复中具有有益效果。然而，大多数电刺激都是通过经皮电极进行的，容易造成严重的创伤。使用非接触式电刺激 (NCES) 有望降低潜在风险。在本研究中，通过自行设计的实用装置方便地发挥了 NCES。通过 EF 分布分析选择间距为 10 cm、边长为 21 和 30 cm 的电极板，分别将 NCES 应用于细胞和小鼠，并测量实际的 EF 强度。负载电压的变化对 EF 分布的规律性没有影响，可作为单因素探讨 NCES 对伤口愈合的影响。随后证明，53 mV mm ^-1NCES促进体外HaCaT细胞和HDF的迁移和增殖，以及巨噬细胞的M2型极化。此外，54和84 mV mm ^-1 NCESs从减少瘢痕形成、增强胶原合成和增加体内血管生成的角度加速了模型小鼠的伤口愈合速度。NCES在伤口愈合中的促进作用显示了为皮肤组织损伤的临床治疗开辟新的可能性的潜力。