A 28-day feeding trial was conducted to evaluate the effects of different dietary preparations of Enterococcus faecalis F7 (blank control (BC), dead bacteria (DB), live bacteria (EF), cell-free extract of E. faecalis F7 (BF) and E. faecalis F7 treated with lithium chloride (Li)) on growth and intestinal microbiota of Litopenaeus vannamei. Results indicated that incorporation of live E. faecalis F7 into diet significantly improved the growth of L. vannamei by increasing weight gain rate (WGR), specific growth rate (SGR) and reducing feed conversion ratio (FCR). High-throughput sequencing data showed that incorporation of various preparations of E. faecalis F7 into diet altered intestinal microbiota richness and diversity in L. vannamei. In particular, live E. faecalis F7 improved the health of shrimp by increasing the abundance of beneficial bacteria and reducing the abundance of detrimental bacteria in the gut tract. Moreover, stability of intestinal microbiota was maintained for more than 7 days when shrimps were fed on basic diet after administration of live E. faecalis F7 for 28 days. These results demonstrate that diet enriched with live E. faecalis F7 promotes the growth and modulates the intestinal microbiota in Pacific white shrimp (L. vannamei).

中文翻译：

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粪肠球菌F7不同日粮制剂对太平洋白对虾（Litopenaeus vannamei）生长和肠道菌群的影响

进行了为期 28 天的喂养试验，以评估粪肠球菌F7 不同膳食制剂（空白对照 (BC)、死菌 (DB)、活菌 (EF)、粪肠球菌 F7 无细胞提取物 ( BF ) 和用氯化锂 (Li) 处理的 粪肠球菌F7) 对南美白对虾生长和肠道微生物群的影响。结果表明，在日粮中添加活粪肠球菌F7 可显着改善L的生长。 南美白通过提高增重率 (WGR)、比生长率 (SGR) 和降低饲料转化率 (FCR)。高通量测序数据显示，掺入了多种大肠杆菌制剂。 粪肠球菌F7 进入饮食改变了L . 南美白 特别是，活粪肠球菌F7 通过增加有益细菌的丰度和减少肠道中有害细菌的丰度来改善虾的健康。此外，当虾在施用活大肠杆菌后以基础饲料喂养时，肠道微生物群的稳定性保持了 7 天以上。 粪便F7 28 天。这些结果表明，富含活粪肠球菌F7 的饮食可促进太平洋白虾（南美白对虾）的生长并调节肠道微生物群。