In order to evaluate the supplemental effects of amino acids and bile acid to completely replace fish meal by enzymatically hydrolysed soybean meal diet for rainbow trout (Oncorhynchus mykiss), a 56-day feeding trial was conducted with five diets, including 40% fish meal (FM) as a positive control diet, 45% enzymatically hydrolysed soybean meal without the fish meal (ESM) as a negative control diet, the addition of methionine (Met) and lysine (Lys) (0.76%) to the ESM diet (E+A), the addition of 0.02% bile acid to ESM diet (E+B) and the addition of Met and Lys (0.76%) and 0.02% bile acid to ESM diet (E+AB). The results showed that the ESM group reduced the growth performance but significantly alleviated the liver health problem, decreased lipid and saturated fatty acids (SFA) contents and increased protein content in fish muscle. The E+A group significantly improved growth performance, pepsin activity and fillet protein content, while the E+B group inhibited growth and protease activity but increased amylase activity, elevated SFA, monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) contents and improved lipid utilization. The E+AB group had superiority in enhancing liver health compared with E+A and E+B groups. This study suggested that supplemental amino acids and bile acid alone or in combination can be used according to practical feeding demand.

中文翻译：

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酶水解豆粕日粮完全替代鱼粉中补充氨基酸和胆汁酸对虹鳟鱼（Oncorhynchus mykiss）生长性能、肝脏健康和鱼片品质的影响

为了评价氨基酸和胆汁酸完全替代酶解豆粕日粮对虹鳟鱼（Oncorhynchus mykiss ）的补充效果。)，用五种日粮进行了为期 56 天的喂养试验，包括 40% 鱼粉 (FM) 作为阳性对照日粮，45% 不含鱼粉的酶水解豆粕 (ESM) 作为阴性对照日粮，添加在 ESM 饮食 (E+A) 中添加蛋氨酸 (Met) 和赖氨酸 (Lys) (0.76%)，在 ESM 饮食 (E+B) 中添加 0.02% 胆汁酸以及添加 Met 和 Lys (0.76%) 和0.02% 胆汁酸到 ESM 饮食 (E+AB)。结果表明，ESM组降低了生长性能，但显着缓解了肝脏健康问题，降低了脂肪和饱和脂肪酸（SFA）含量，增加了鱼肌肉中的蛋白质含量。E+A 组显着提高了生长性能、胃蛋白酶活性和鱼片蛋白含量，而 E+B 组抑制了生长和蛋白酶活性，但增加了淀粉酶活性，提高了 SFA、单不饱和脂肪酸 (MUFA) 和多不饱和脂肪酸 (PUFA) 含量和改善脂质利用率。与 E+A 和 E+B 组相比，E+AB 组在增强肝脏健康方面具有优势。本研究提示可根据实际饲养需求单独或联合使用补充氨基酸和胆汁酸。