Xylella fastidiosa is a xylem-limited bacterium native to America and classified as a priority pest for EU regulation. Since 2013, X. fastidiosa has been identified in European countries with a Mediterranean climate, such as Italy, France, Spain and Portugal, with different subspecies and sequence types (ST) detected. Since 2015 X. fastidiosa subsp. multiplex ST6 and/or ST7 has been detected in Corsica and the Provence-Alpes-Côte d’Azur in almost 70 plant species, whereas X. fastidiosa subsp. pauca ST53 has been found in only two host plants. In this study, we report two new variants, recently detected in two separated areas of the PACA region, genetically related to the subspecies multiplex and assigned to (i) ST88 detected on Polygala myrtifolia, Hebe sp., Osteospermum ecklonis, Lavandula x intermedia, Coronilla glauca and Euryops chrysanthemoides and (ii) ST89: detected on Myoporum sp. and Viburnum tinus. Both variant strains were isolated in vitro. Moreover, we report here the identification of X. fastidiosa subsp. multiplex ST6 in a new region of the South of France, Occitanie (Aude), in plants from natural and urban settings and from a nursery.

Xylella fastidiosa是一种原产于美国的木质部限制性细菌，被归类为欧盟法规的重点害虫。自 2013 年以来，在地中海气候的欧洲国家，如意大利、法国、西班牙和葡萄牙，已经发现了X. fastidiosa ，并检测到了不同的亚种和序列类型 (ST)。自 2015 年以来X. fastidiosa subsp. 在科西嘉岛和普罗旺斯-阿尔卑斯-蔚蓝海岸的近 70 种植物中检测到多重ST6 和/或 ST7，而X. fastidiosa subsp。仅在两种寄主植物中发现了pauca ST53。在这项研究中，我们报告了两个新变种，最近在 PACA 区域的两个分离区域中检测到，与亚种遗传相关多路复用并分配给 (i) ST88 在远志、白鹤芋、Osteospermum ecklonis 、 Lavandula x intermedia、Coronilla glauca和Euryops chrysanthemoides上检测到，以及 (ii) ST89：在Myoporum sp.上检测到。和Viburnum tinus。两种变体菌株均在体外分离。此外，我们在这里报告了X. fastidiosa亚种的鉴定。在法国南部的一个新地区 Occitanie (Aude) 使用来自自然和城市环境以及苗圃的植物中的多重ST6。