Perennial grain crops have been proposed as a groundbreaking tactic to establish sustainable agroecosystems, as their growth habit and no-till management likely invoke multifunctionality such as improvement of soil structure as well as increases in nutrient retention and carbon (C) sequestration. Arbuscular mycorrhizal fungi (AMF) are multifunctional symbioses, which are ecologically important for over 80% terrestrial plants that form root associations with these fungi. Our study examined AMF diversity and community composition in annual and perennial grain crops; with and without nitrogen (N) fertilizer addition at two field sites within the Canadian prairies (Breton and Edmonton). AMF diversity and community composition were significantly different between the two study sites. This was attributed to the contrasting cropping management histories of the sites; previous long-term land use in Edmonton was annual cropping, whereas Breton was perennial forage crop. AMF community composition was not different between annual versus perennial grain cropping on both the bulk soil and rhizosphere (roots). Overall, the addition of N fertilizer did not change AMF diversity and community compositions but increased the abundance of Archaeospora. The most predominant genus in these temperate agroecosystems was Paraglomus, while Archaeospora and Claroideoglomus were observed in the roots compartment but unfrequently and specifically at the Edmonton site. Interestingly, Archaeospora and Paraglomus were significantly correlated with changes in particulate organic matter C, while Claroideoglomus had a positive correlation with ammonium concentration. Results revealed functional associations between key soil properties and certain AMF genera, which showed to be site specific.

多年生粮食作物已被提议作为建立可持续农业生态系统的开创性策略，因为它们的生长习性和免耕管理可能会引发多功能性，例如改善土壤结构以及增加养分保留和碳 (C) 封存。丛枝菌根真菌 (AMF) 是多功能共生体，对 80% 以上与这些真菌形成根系的陆生植物具有重要的生态学意义。我们的研究检查了一年生和多年生粮食作物的 AMF 多样性和群落组成；在加拿大大草原（布列塔尼和埃德蒙顿）的两个田间地点添加和不添加氮 (N) 肥料。两个研究地点之间的 AMF 多样性和群落组成存在显着差异。这归因于这些地点不同的种植管理历史；埃德蒙顿以前的长期土地使用是一年生作物，而布列塔尼是多年生草料作物。AMF 群落组成在大块土壤和根际（根）上的一年生和多年生谷物种植之间没有差异。总体而言，氮肥的添加并未改变 AMF 多样性和群落组成，但增加了 AMF 的丰度。古孢子菌。在这些温带农业生态系统中，最主要的属是Paraglomus，而在根隔室中观察到Archaeospora和Clroideoglomus ，但在埃德蒙顿地区很少见且特别明显。有趣的是，Archaaeospora和Paraglomus与颗粒有机物 C 的变化显着相关，而Clroideoglomus与铵浓度呈正相关。结果揭示了关键土壤特性和某些 AMF 属之间的功能关联，这表明是特定地点的。