Roles of oral microbiota and oral-gut microbial transmission in hypertension,Journal of Advanced Research

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Roles of oral microbiota and oral-gut microbial transmission in hypertension
Journal of Advanced Research ( IF 11.4 ) Pub Date : 2022-03-19 , DOI: 10.1016/j.jare.2022.03.007
Bo-Yan Chen ₁ , Wen-Zhen Lin ₂ , Yu-Lin Li ₁ , Chao Bi ₃ , Lin-Juan Du ₁ , Yuan Liu ₁ , Lu-Jun Zhou ₂ , Ting Liu ₁ , Shuo Xu ₁ , Chao-Ji Shi ₄ , Hong Zhu ₁ , Yong-Li Wang ₁ , Jian-Yong Sun ₁ , Yan Liu ₁ , Wu-Chang Zhang ₁ , Hai-Xia Lu ₅ , Yi-Hua Wang ₆ , Qiang Feng ₆ , Fu-Xiang Chen ₇ , Chang-Qian Wang ₈ , Maurizio S Tonetti ₉ , Ya-Qin Zhu ₁₀ , Huili Zhang ₈ , Sheng-Zhong Duan ₁

Affiliation

Laboratory of Oral Microbiota and Systemic Diseases, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology, Shanghai, China.
Laboratory of Oral Microbiota and Systemic Diseases, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology, Shanghai, China; Department of General Dentistry, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Stomatology, First Affiliated Hospital, Anhui Medical University, Hefei, China.
National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology, Shanghai, China; Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology, Shanghai, China; Department of Preventive Dentistry, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Human Microbiome, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, China.
Department of Clinical Immunology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Cardiology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology, Shanghai, China; Department of Oral and Maxillofacial Implantology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology, Shanghai, China; Department of General Dentistry, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Introduction

Considerable evidence has linked periodontitis (PD) to hypertension (HTN), but the nature behind this connection is unclear. Dysbiosis of oral microbiota leading to PD is known to aggravate different systematic diseases, but the alteration of oral microbiota in HTN and their impacts on blood pressure (BP) remains to be discovered.

Objectives

To characterize the alterations of oral and gut microbiota and their roles in HTN.

Methods

We performed a cross-sectional (95 HTN participants and 39 controls) and a 6-month follow-up study (52 HTN participants and 26 controls) to analyze the roles of oral and gut microbiota in HTN. Saliva, subgingival plaques, and feces were collected for 16S rRNA gene sequencing or metagenomic analysis. C57BL/6J mice were pretreated with antibiotics to deplete gut microbiota, and then transplanted with human saliva by gavage to test the impacts of abnormal oral-gut microbial transmission on HTN.

Results

BP in participants with PD was higher than no PD in both cross-sectional and follow-up cohort. Relative abundances of 14 salivary genera, 15 subgingival genera and 10 gut genera significantly altered in HTN and those of 7 salivary genera, 12 subgingival genera and 6 gut genera significantly correlated with BP. Sixteen species under 5 genera were identified as oral-gut transmitters, illustrating the presence of oral-gut microbial transmission in HTN. Veillonella was a frequent oral-gut transmitter stably enriched in HTN participants of both cross-sectional and follow-up cohorts. Saliva from HTN participants increased BP in hypertensive mice. Human saliva-derived Veillonella successfully colonized in mouse gut, more abundantly under HTN condition.

Conclusions

PD and oral microbiota are strongly associated with HTN, likely through oral-gut transmission of microbes. Ectopic colonization of saliva-derived Veillonella in the gut may aggravate HTN. Therefore, precise manipulations of oral microbiota and/or oral-gut microbial transmission may be useful strategies for better prevention and treatment of HTN.

中文翻译：

口腔微生物群和口腔-肠道微生物传播在高血压中的作用

介绍

大量证据表明牙周炎 (PD) 与高血压 (HTN) 相关，但这种联系背后的本质尚不清楚。已知导致 PD 的口腔微生物群失调会加重不同的系统性疾病，但 HTN 中口腔微生物群的改变及其对血压 (BP) 的影响仍有待发现。

目标

描述口腔和肠道微生物群的变化及其在 HTN 中的作用。

方法

我们进行了一项横断面研究（95 名 HTN 参与者和 39 名对照组）和一项为期 6 个月的随访研究（52 名 HTN 参与者和 26 名对照组）来分析口腔和肠道微生物群在 HTN 中的作用。收集唾液、龈下菌斑和粪便进行 16S rRNA 基因测序或宏基因组分析。C57BL/6J 小鼠用抗生素预处理以耗尽肠道微生物群，然后通过管饲法移植人类唾液以测试异常口腔-肠道微生物传播对 HTN 的影响。

结果

在横断面和随访队列中，患有 PD 的参与者的血压均高于无 PD 的血压。14个唾液属、15个龈下属和10个肠道属的相对丰度在HTN中显着改变，7个唾液属、12个龈下属和6个肠道属的相对丰度与BP显着相关。5 个属下的 16 个物种被确定为口腔肠道递质，说明 HTN 中存在口腔-肠道微生物传播。Veillonella是一种常见的口腔肠道递质，在横断面和随访队列的 HTN 参与者中稳定富集。来自 HTN 参与者的唾液增加了高血压小鼠的血压。人唾液来源的韦荣球菌成功地定殖在小鼠肠道中，在 HTN 条件下更为丰富。