This study investigated the effects of 7 days of 600 mg/day anthocyanin-rich blackcurrant extract intake on small intestinal permeability, enterocyte damage, microbial translocation, and inflammation following exertional heat stress. Twelve recreationally active men (maximal aerobic capacity = 55.6 ± 6.0 ml·kg⁻¹·min⁻¹) ran (70% VO₂max) for 60 min in an environmental chamber (34 °C, 40% relative humidity) on two occasions (placebo/blackcurrant, randomized double-blind crossover). Permeability was assessed from a 4-hr urinary excretion of lactulose and rhamnose and expressed as a ratio of lactulose/rhamnose. Venous blood samples were taken at rest and 20, 60, and 240 min after exercise to measure enterocyte damage (intestinal fatty acid-binding protein); microbial translocation (soluble CD14, lipopolysaccharide-binding protein); and interleukins 6, interleukins 10, and interleukins 1 receptor antagonist. Exercise increased rectal temperature (by ∼2.8 °C) and heart rate (by ∼123 beats/min) in each condition. Blackcurrant supplementation led to a ∼12% reduction in lactulose/rhamnose ratio (p < .0034) and enterocyte damage (∼40% reduction in intestinal fatty acid-binding protein area under the curve; p < .0001) relative to placebo. No between-condition differences were observed immediately after exercise for lipopolysaccharide-binding protein (mean, 95% confidence interval [CI]; +80%, 95% CI [+61%, +99%]); soluble CD14 (+37%, 95% CI [+22%, +51%]); interleukins 6 (+494%, 95% CI [+394%, +690%]); interleukins 10 (+288%, 95% CI [+105%, +470%]); or interleukins 1 receptor antagonist (+47%, 95% CI [+13%, +80%]; all time main effects). No between-condition differences for these markers were observed after 60 or 240 min of recovery. Blackcurrant extract preserves the GI barrier; however, at subclinical levels, this had no effect on microbial translocation and downstream inflammatory processes.

本研究调查了每天 7 天摄入 600 毫克富含花青素的黑加仑提取物对运动性热应激后小肠通透性、肠细胞损伤、微生物易位和炎症的影响。12 名从事休闲活动的男性（最大有氧能力 = 55.6 ± 6.0 ml·kg ^-1 ·min ^-1）跑步（70% VO ₂最大）在环境室（34 °C，40% 相对湿度）中两次（安慰剂/黑醋栗，随机双盲交叉）60 分钟。从乳果糖和鼠李糖的 4 小时尿排泄中评估渗透性，并表示为乳果糖/鼠李糖的比率。在静息和运动后 20、60 和 240 分钟采集静脉血样，以测量肠细胞损伤（肠脂肪酸结合蛋白）；微生物易位（可溶性 CD14，脂多糖结合蛋白）；白细胞介素 6、白细胞介素 10 和白细胞介素 1 受体拮抗剂。在每种情况下，运动都会增加直肠温度（约 2.8 °C）和心率（约 123 次/分钟）。黑醋栗补充剂导致乳果糖/鼠李糖比例降低约 12%（p < .0034）和肠细胞损伤（曲线下肠道脂肪酸结合蛋白面积减少约 40%；p  < .0001）相对于安慰剂。运动后立即没有观察到脂多糖结合蛋白的条件间差异（平均，95% 置信区间 [CI]；+80%，95% CI [+61%，+99%]）；可溶性 CD14 (+37%, 95% CI [+22%, +51%]); 白细胞介素 6 (+494%, 95% CI [+394%, +690%]); 白细胞介素 10 (+288%, 95% CI [+105%, +470%]); 或白细胞介素 1 受体拮抗剂（+47%，95% CI [+13%，+80%]；所有时间的主要影响）。在恢复 60 或 240 分钟后，未观察到这些标志物的条件间差异。黑加仑提取物可保护胃肠道屏障；然而，在亚临床水平上，这对微生物易位和下游炎症过程没有影响。