Chitosan is a well-known polymer widely used in tissue engineering and regenerative medicine. It is biocompatible, biodegradable, non-toxic, has antibacterial and osteoconductive properties. Chitosan is often used in the form of composites (with the participation of ceramic particles), membranes, hydrogels or nanoparticles. The problem with biomaterials is their low durability, rapid degradation, poor mechanical properties and cytotoxicity. Cross-linking or stabilization of such materials allows for solving these problems. It is important that the compounds used for this purpose exhibit limited or no toxicity. The presented article is a review and presents some methods of cross-linking/stabilization of chitosan structures. The analysis concerns low or non-cytotoxic cross-linking/stabilization methods. The discussed compounds used for the purpose of chitosan structure fixation are: cinnamaldehyde, genipin, L-aspartic acid, vanillin, sodium carbonate, sodium alginate, BGP, ethanol and TPP. There is discussed also a hydrothermal/dehydrothermal method which seems to be promising as it is more advantageous since no additional compounds are introduced into the structure.

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用于潜在医学应用的壳聚糖结构的交联和稳定方法

壳聚糖是一种广为人知的聚合物，广泛用于组织工程和再生医学。它具有生物相容性、可生物降解、无毒、抗菌和骨传导特性。壳聚糖通常以复合材料（有陶瓷颗粒参与）、膜、水凝胶或纳米颗粒的形式使用。生物材料的问题是它们的低耐久性、快速降解、差的机械性能和细胞毒性。这种材料的交联或稳定化可以解决这些问题。重要的是，用于此目的的化合物表现出有限的毒性或没有毒性。本文是一篇综述，并介绍了一些交联/稳定壳聚糖结构的方法。该分析涉及低或无细胞毒性的交联/稳定方法。讨论的用于固定壳聚糖结构的化合物有：肉桂醛、京尼平、L-天冬氨酸、香草醛、碳酸钠、海藻酸钠、BGP、乙醇和 TPP。还讨论了一种似乎很有前途的水热/脱水热方法，因为它更有利，因为没有将额外的化合物引入结构中。