Canopy bridges are crossing structures specific to mitigate the impact of roads on arboreal animals. Long-term monitoring of such infrastructures together with the analysis of design preferences has never been done in South America. To avoid the roadkills of a threatened primate species, the black lion tamarin (Leontopithecus chrysopygus), in Guareí, São Paulo, Brazil, we installed two designs of canopy bridges: a wood pole bridge and a rope bridge. We aimed to (1) evaluate the functionality (number of species and events) of both designs, (2) test the design preference of each species, and (3) determine if there were seasonal differences in the use of canopy bridges. We monitored the canopy bridges continuously since their installation with camera traps during 3 years. We recorded nine mammal and one lizard species crossing on the canopy bridges as well as 13 bird species using them as perches. Overall, the probability of crossing was higher on the wood pole bridge and the number of crossings, considering both designs, was higher during the dry season. One lizard and seven mammal species used the wood pole bridge, including the black lion tamarin, and six mammal species used the rope bridge. Four out of five species tested, including the black lion tamarin, preferred the wood pole bridge. While replications of this experimental design are necessary to obtain a more robust evaluation of the effectiveness of these canopy bridges, our study suggests that wood pole bridges might be an effective tool to reduce roadkills of the endangered black lion tamarin and possibly other arboreal species.

天篷桥是专门用于减轻道路对树栖动物影响的交叉结构。南美洲从未对此类基础设施进行长期监测以及对设计偏好的分析。为了避免濒临灭绝的灵长类动物，黑狮狨（Leontopithecus chrysopygus)，在巴西圣保罗的瓜雷里，我们安装了两种设计的天篷桥：木杆桥和绳桥。我们旨在 (1) 评估两种设计的功能（物种和事件的数量），(2) 测试每个物种的设计偏好，以及 (3) 确定树冠桥的使用是否存在季节性差异。自安装了摄像头陷阱以来，我们在 3 年内不断监测天篷桥。我们记录了 9 种哺乳动物和 1 种蜥蜴物种在树冠桥上穿越，以及 13 种鸟类将它们用作栖息地。总体而言，木杆桥的穿越概率较高，考虑到两种设计，旱季穿越的次数也较高。一种蜥蜴和七种哺乳动物使用了木杆桥，包括黑狮狨猴，六种哺乳动物使用了绳桥。五分之四的测试物种，包括黑狮狨猴，更喜欢木杆桥。虽然重复该实验设计对于更可靠地评估这些树冠桥的有效性是必要的，但我们的研究表明，木杆桥可能是减少濒临灭绝的黑狮狨猴和可能的其他树栖物种的道路杀伤力的有效工具。