Previous studies showed that chicken manure and straws can be used as a feedstock in the production of methane during anaerobic digestions. The objective was to determine the best mixture of chicken manure and straw in the production of methane during anaerobic digestion. In this study the effect of three volatile solid (VS) rations on methane output, pH, hydrolase activity, and microbial community structure was determined. The optimal manure–straw VS ratio was M2 (9:1), where cumulative methane output reached a maximum of 227,660 ml kg^–1 in 50 d. The cellulose activity was higher in the chicken manure to straw ratio of 9 than 4 or 1. The research showed that Megasphaera and Bacteroides were important cellulose-secreting bacteria. The results show: (a) during anaerobic digestion the cellulose and lignin contents were completely degraded; (b) protease, amylase, and lipase activities were higher in M2 (9:1) than M1 (1:0) or M3 (4:1); and (c) increasing amount of straw decreased bacteria and archaea diversity. Due to the addition of straw, the unidentified_Prevotellaceae had good physiological activity in the acidic environment between 5 and 30 d, Candidatus_Cloacimonas, unidentified_Lentimicrobiaceae, and Ruminiclostridium were the main archaea between 40 and 50 d.

中文翻译：

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秸秆和鸡粪厌氧共消化产甲烷及微生物群落特征

先前的研究表明，鸡粪和秸秆可用作厌氧消化过程中产生甲烷的原料。目的是确定在厌氧消化过程中产生甲烷的鸡粪和稻草的最佳混合物。在这项研究中，确定了三种挥发性固体 (VS) 口粮对甲烷输出、pH、水解酶活性和微生物群落结构的影响。最佳粪肥与秸秆 VS 比为 M2 (9:1)，其中 50 d 内累积甲烷输出量达到最大值 227,660 ml kg ^–1。鸡粪秸秆比为 9 时纤维素活性高于 4 或 1。研究表明，Megasphaera和Bacteroides是重要的纤维素分泌细菌。结果表明： (a) 在厌氧消化过程中，纤维素和木质素含量被完全降解；(b) M2 (9:1) 中的蛋白酶、淀粉酶和脂肪酶活性高于 M1 (1:0) 或 M3 (4:1)；(c) 秸秆数量的增加会降低细菌和古细菌的多样性。由于秸秆的添加，未鉴定的_普雷沃菌科在酸性环境中5~30 d具有良好的生理活性，念珠菌_漏斗孢菌属、未鉴定的_扁豆菌科、瘤胃梭菌是40~50 d的主要古菌。