Chinese medicine extracts are complex in composition. The combination of the quantitative analysis of multicomponents by single marker (QAMS) and the systematic quantified fingerprint method (SQFM) can be used for better quantitative analysis. The contents of Pulsatilla saponin D, Pulsatilla saponin A, Pulsatilla saponin F, and oleanolic acid 3-o-β-d-pyranoglucosyl-(1⟶4)-β-d-pyranoglucosyl-(1⟶3)-α-l-pyridine rhamnosyl-(1⟶2)-α-l-pyranosine arabinoside (B9) were determined by HPLC and QAMS. The methodological verification was carried out. The relative correction factor (RCF) was calculated, and the reproducibility of the RCF was investigated. The experimental results of the external standard method (ESM) and the QAMS were compared. Meanwhile, the fingerprint of the extract of Pulsatilla chinensis total saponins was established and the quality of the extract was evaluated by SQFM and hierarchical cluster analysis (HCA). The results showed that there was no significant difference between the QAMS and ESM. QAMS could be used for the rapid determination of various saponins in the extract of Pulsatilla chinensis. SQFM and HCA could objectively and comprehensively reflect the overall quality difference of total saponin extract of Pulsatilla chinensis. Therefore, QAMS and SQFM could provide a more convenient and effective selection for the quality evaluation of total saponin extract from this plant.

中文翻译：

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单标记法结合系统定量指纹法多组分定量分析白头翁总皂苷提取物质量评价

中药提取物成分复杂。多组分单标记定量分析（QAMS）与系统量化指纹法（SQFM）相结合，可以更好地进行定量分析。白头翁皂甙D、白头翁皂甙A、白头翁皂甙F、齐墩果酸3-o- β -d-吡喃葡萄糖基-(1⟶4) -β -d-吡喃葡萄糖基-(1⟶3) -α - l-的含量吡啶鼠李糖基-(1⟶2) -α-l-吡喃糖苷阿拉伯糖苷(B9)通过HPLC和QAMS测定。进行了方法学验证。计算了相对校正因子（RCF），并研究了RCF的重现性。比较了外标法（ESM）和QAMS的实验结果。同时，建立了白头翁总皂苷提取物指纹图谱，采用SQFM和层次聚类分析（HCA）对提取物质量进行评价。结果表明，QAMS 和 ESM 之间没有显着差异。QAMS可用于白头翁提取物中各种皂苷的快速测定. SQFM和HCA能客观、全面地反映白头翁总皂苷提取物的整体质量差异。因此，QAMS和SQFM可以为该植物总皂苷提取物的质量评价提供更方便、更有效的选择。