Background Circular RNA (circRNA) plays key regulatory roles in the development of many diseases. However the biological functions and potential molecular mechanisms of circRNA in the injury and repair of intestinal mucosa in mice after severe burns are yet to be elucidated. Methods Cell counting kit-8 (CCK-8), 5-ethynyl-2′-deoxyuridine (EdU), wound healing and transwell assays were used to detect cell proliferation and migration ability. Real-time quantitative PCR was used to identify the expression of circRNA, microRNA and messenger RNA. Nuclear and cytoplasmic separation experiments were employed to perceive the location of circRNA_Maml2. Finally, in vitro and in vivo experiments were conducted to study the repairing effect of circRNA_Maml2 on the intestinal mucosa of mice after severe burns. Results When compared with the control group, the expression of circRNA_Maml2 was significantly reduced in the severe burn group. Furthermore, overexpression of circRNA_Maml2 promoted the proliferation and migration of CT26.wt cells in vivo and the repair of damaged intestinal mucosa in vitro. CircRNA_Maml2 acted as a sponge adsorption molecule for miR-93-3p to enhance the expression of frizzled class receptor 7 and activate the downstream Wnt/β-catenin pathway, thereby promoting the repair of the intestinal mucosa. Conclusions Our findings demonstrate that circRNA_Maml2 regulates the miR-93-3p/FZD7/Wnt/β-catenin pathway and promotes the repair of damaged intestinal mucosa. Hence, circRNA_Maml2 is a potential therapeutic target to promote intestinal mucosal repair.

中文翻译：

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CircRNA_Maml2通过调控miR-93-3p/FZD7/Wnt/β-catenin通路促进严重烧伤后肠上皮细胞的增殖和迁移

背景 环状 RNA (circRNA) 在许多疾病的发展中起着关键的调控作用。但circRNA在小鼠严重烧伤后肠黏膜损伤修复中的生物学功能及潜在分子机制尚待阐明。方法细胞计数试剂盒8（CCK-8）、5-乙炔基-2'-脱氧尿苷（EdU）、伤口愈合和transwell试验检测细胞增殖和迁移能力。实时定量PCR用于鉴定circRNA、microRNA和信使RNA的表达。采用核和细胞质分离实验来感知 circRNA_Maml2 的位置。最后通过体外和体内实验研究circRNA_Maml2对小鼠严重烧伤后肠黏膜的修复作用。结果与对照组相比，严重烧伤组circRNA_Maml2的表达明显降低。此外，circRNA_Maml2的过表达促进了CT26.wt细胞在体内的增殖和迁移以及体外受损肠黏膜的修复。CircRNA_Maml2作为miR-93-3p的海绵吸附分子，增强frizzled class receptor 7的表达，激活下游Wnt/β-catenin通路，从而促进肠黏膜的修复。结论 我们的研究结果表明，circRNA_Maml2 调节 miR-93-3p/FZD7/Wnt/β-catenin 通路，促进受损肠黏膜的修复。因此，circRNA_Maml2 是促进肠黏膜修复的潜在治疗靶点。circRNA_Maml2的过表达促进了CT26.wt细胞在体内的增殖和迁移以及体外受损肠黏膜的修复。CircRNA_Maml2作为miR-93-3p的海绵吸附分子，增强frizzled class receptor 7的表达，激活下游Wnt/β-catenin通路，从而促进肠黏膜的修复。结论 我们的研究结果表明，circRNA_Maml2 调节 miR-93-3p/FZD7/Wnt/β-catenin 通路，促进受损肠黏膜的修复。因此，circRNA_Maml2 是促进肠黏膜修复的潜在治疗靶点。circRNA_Maml2的过表达促进了CT26.wt细胞在体内的增殖和迁移以及体外受损肠黏膜的修复。CircRNA_Maml2作为miR-93-3p的海绵吸附分子，增强frizzled class receptor 7的表达，激活下游Wnt/β-catenin通路，从而促进肠黏膜的修复。结论 我们的研究结果表明，circRNA_Maml2 调节 miR-93-3p/FZD7/Wnt/β-catenin 通路，促进受损肠黏膜的修复。因此，circRNA_Maml2 是促进肠黏膜修复的潜在治疗靶点。CircRNA_Maml2作为miR-93-3p的海绵吸附分子，增强frizzled class receptor 7的表达，激活下游Wnt/β-catenin通路，从而促进肠黏膜的修复。结论 我们的研究结果表明，circRNA_Maml2 调节 miR-93-3p/FZD7/Wnt/β-catenin 通路，促进受损肠黏膜的修复。因此，circRNA_Maml2 是促进肠黏膜修复的潜在治疗靶点。CircRNA_Maml2作为miR-93-3p的海绵吸附分子，增强frizzled class receptor 7的表达，激活下游Wnt/β-catenin通路，从而促进肠黏膜的修复。结论 我们的研究结果表明，circRNA_Maml2 调节 miR-93-3p/FZD7/Wnt/β-catenin 通路，促进受损肠黏膜的修复。因此，circRNA_Maml2 是促进肠黏膜修复的潜在治疗靶点。