Aquaculture International ( IF 2.2 ) Pub Date : 2022-03-07 , DOI: 10.1007/s10499-022-00862-2 Qian Liu 1 , Junnan Li 1 , Hongwei Shan 1 , Yicheng Xie 1 , Dongxu Zhang 2
|
The integrated multitrophic aquaculture (IMTA) pond represents a unique ecosystem in which various species of aquatic organisms with different trophic levels are co-cultured together, achieving ecological and economic effects by using ecological principles. This study was designed to investigate the composition pattern of the bacterial communities and potential drivers in IMTA ponds of Penaeus japonicus-Portunus trituberculatus (SC) and P. japonicus-P. trituberculatus-Sinonovacula constricta (SCC). SC and SCC sediments were collected in July, October, and December and used to sequence the 16S rRNA gene and analyze physical and chemical indicators. The α diversity of the bacterial community was not significantly different between the sediments of SC and SCC. The dominant phyla of bacterial communities in sediments of SC and SCC shared a large number of common members. Bacterial members involved in denitrification were biomarkers in the sediment of SC, while biomarkers involved in nitrification, assimilatory nitrate reduction, and nitrogen fixation were found in the sediment of SCC. The β diversity was significantly different between SC and SCC. The bacterial community composition dissimilarity was significantly correlated with environmental factors, which were driven by pH, NH4+ concentration in the sediment (S_ NH4+), and NO2− concentration in the sediment (S_ NO2−) in July, and pH was the most important explanatory variable for the community composition dissimilarity (P < 0.05). The dissimilarity was driven by oxidation–reduction potential (ORP) and NO2− concentration in the pore water of sediments (P_ NO2−) in October, and P_ NO2− was the most important variable (P < 0.05). The S_ NH4+, S_ NO2−, and pH exhibited interactions for the dissimilarity in December, and the S_ NH4+ was the most important variable (P < 0.05). Additionally, the pH was significantly correlated with the S_ NH4+ and S. constricta densities (P < 0.05). These results indicated that the co-culture of S. constricta in shrimp-crab ponds may cause specific composition patterns of bacterial communities by influencing pH and inorganic nitrogen sources, resulting in an increase in bacterial mineralization and nutrient catabolism and a shift in the nitrogen cycling processes from denitrification to nitrification, assimilatory nitrate reduction, and nitrogen fixation in the sediment.
Graphical abstract
中文翻译：
两种典型的综合多营养水产养殖系统沉积物中细菌群落的特定模式和驱动因素
综合多营养水产养殖（IMTA）池塘代表了一个独特的生态系统，其中不同营养级的各种水生生物共同养殖，利用生态学原理实现生态和经济效应。本研究旨在调查 IMTA 池塘中日本对虾-三结核梭子蟹(SC) 和日本对虾- 三结核对虾 - Sinonovacula constricta的细菌群落组成模式和潜在驱动因素。(SCC)。7、10、12月采集SC和SCC沉积物，用于16S rRNA基因测序和理化指标分析。SC和SCC沉积物中细菌群落的α多样性没有显着差异。SC和SCC沉积物中细菌群落的优势门共享大量共同成员。参与反硝化的细菌成员是SC沉积物中的生物标志物，而SCC沉积物中参与硝化、同化硝酸盐还原和固氮的生物标志物。SC和SCC之间的β多样性显着不同。细菌群落组成差异与环境因素显着相关，环境因素受 pH、NH 4 +驱动7 月份沉积物中的浓度（S_ NH 4 +）和沉积物中的 NO 2 -浓度（S_ NO 2 -），pH 是群落组成差异最重要的解释变量（P < 0.05）。10月份沉积物孔隙水中的氧化还原电位（ORP）和NO 2 -浓度（P_ NO 2 -）驱动了这种差异，其中P_ NO 2 -是最重要的变量（P <0.05）。S_ NH 4 + , S_ NO 2 -, 和 pH 对 12 月的差异表现出交互作用，其中 S_ NH 4 +是最重要的变量（P < 0.05）。此外，pH与S_ NH 4 +和S. constricta密度显着相关( P < 0.05)。这些结果表明，虾蟹池中的S. constricta共培养可能通过影响 pH 值和无机氮源导致细菌群落的特定组成模式，从而导致细菌矿化和营养分解代谢的增加以及氮循环的转变从反硝化到硝化、同化硝酸盐还原和沉积物中固氮的过程。
京公网安备 11010802027423号