P450 (cytochrome P450) is a supergene family, which is involved in various metabolic pathways in plants. Based on previous study, we found some of cucumber P450 mRNAs were systemic mobile in cucumber/pumpkin grafts. However, the reason that why P450 mRNAs were endorsed as signaling, and what specific motif (s) did they harbored is not clear yet. Here, we first identified 221 CsaP450 genes in cucumber genome-wide level. Combining with graft-transmissible mRNAs datasets in cucumber, we elucidated 15 mobile-CsaP450-coding genes, of which 5 and 10 belonged to A-type and non-A type respectively. Compared with Arabidopsis and pumpkin (Cucubit moschata) graft-induced-transmissible P450 mRNAs, a phylogenetic tree was constructed and divided into eight clans by using multiple-sequence alignment. Gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations indicated that the expression patterns of the mobile-mRNA-coding CsaP450 genes in different tissues of cucumber was specifically enriched in oxidoreductase activity and secondary metabolic pathways. The structures and motifs of these 15 mobile-mRNA-coding CsaP450 genes and their types of regulatory elements told that the proportion of CU-rich motifs was higher than nonmobile-mRNA-coding CsaP450 genes. The integrated analysis of mobility direction and mRNA abundance of 15 mobile-mRNA-coding CsaP450 genes allowed to conclude that there was rarely relationship between them. The study provided a new insight into the relationship between the motifs and functional characterization of mobile-mRNA-coding P450 genes of cucumber in genome-wide levels.

P450（cytochrome P450）是一个超基因家族，参与植物的多种代谢途径。基于之前的研究，我们发现一些黄瓜P450 mRNAs 在黄瓜/南瓜移植物中是系统移动的。然而，为什么P450 mRNA 被认可为信号，以及它们携带什么特定基序的原因尚不清楚。在这里，我们首先在黄瓜全基因组水平上鉴定了 221 个CsaP450基因。结合黄瓜移植物可传播的 mRNA 数据集，我们阐明了 15 个可移动CsaP450编码基因，其中 5 个和 10 个分别属于 A 型和非 A 型。与拟南芥和南瓜（Cucubit moschata）移植物诱导传播相比P450 mRNAs，一个系统发育树被构建并通过使用多序列比对分为八个氏族。基因本体论 (GO) 富集和京都基因与基因组百科全书 (KEGG) 注释表明，可移动 mRNA 编码的 CsaP450 基因在黄瓜不同组织中的表达模式在氧化还原酶活性和次级代谢途径方面特别富集。这 15 个移动 mRNA 编码的CsaP450基因的结构和基序及其调控元件的类型表明富含 CU 的基序的比例高于非移动 mRNA 编码的CsaP450基因。15个mobile-mRNA编码的CsaP450的迁移方向和mRNA丰度的综合分析基因允许得出它们之间很少有关系的结论。该研究为黄瓜移动 mRNA 编码P450基因在全基因组水平上的基序与功能表征之间的关系提供了新的见解。