Purpose

We tend to evaluated a method for loading 6Mercaptopurine (6MP) on green synthesized hybrid chitosan gold nanoparticles (AuNPs) forming 6MP-AuNPs nanocomposite for the first time in combinatorial chemo-photothermal therapy.

Methods

The AuNPs were synthesized using chitosan as a reducing and capping agent. Different concentrations of 6MP were mixed AuNPs. Cells were incubated with 6MP and 6MP loaded AuNPs for 48 h and then exposed to laser.

Results

AuNPs and 6MP-AuNPs nanocomposite have small sizes of 18 ± 4 and 25 ± 5 nm and exhibit high stability with Zeta potential of 55.9 ± 6.3 and 57 ± 4 mV. 6MP-AuNPs nanocomposite irradiated with Diode Pumped Solid State (DPSS) laser showed a maximum inhibition in cell viability reaching 63% at 1.25 µM.

Conclusions

A hybrid chitosan gold nanoparticle is a powerful anti-cancer drug carrier as well as photothermal agent in combinatorial chemo-photothermal therapy.

中文翻译：

---

6巯基嘌呤绿色合成金纳米粒子激光增强联合化学光热治疗乳腺癌模型

目的

我们倾向于在组合化学光热疗法中首次评估将 6Mercaptopurine (6MP) 负载到绿色合成的杂化壳聚糖金纳米粒子 (AuNPs) 上形成 6MP-AuNPs 纳米复合材料的方法。

方法

使用壳聚糖作为还原剂和封端剂合成了 AuNPs。不同浓度的 6MP 是混合的 AuNPs。将细胞与 6MP 和 6MP 负载的 AuNP 一起孵育 48 小时，然后暴露于激光下。

结果

AuNPs 和 6MP-AuNPs 纳米复合材料具有 18 ± 4 和 25 ± 5 nm 的小尺寸，并表现出高稳定性，Zeta 电位为 55.9 ± 6.3 和 57 ± 4 mV。用二极管泵浦固态 (DPSS) 激光照射的 6MP-AuNPs 纳米复合材料在 1.25 µM 时对细胞活力的最大抑制达到 63%。

结论

杂化壳聚糖金纳米粒子是一种强大的抗癌药物载体，也是化学-光热联合治疗中的光热剂。