Ageing is a complex process with common and distinct features across tissues. Unveiling the underlying processes driving ageing in individual tissues is indispensable to decipher the mechanisms of organismal longevity. Caenorhabditis elegans is a well-established model organism that has spearheaded ageing research with the discovery of numerous genetic pathways controlling its lifespan. However, it remains challenging to dissect the ageing of worm tissues due to the limited description of tissue pathology and access to tissue-specific molecular changes during ageing. In this study, we isolated cells from five major tissues in young and old worms and profiled the age-induced transcriptomic changes within these tissues. We observed a striking diversity of ageing across tissues and identified different sets of longevity regulators therein. In addition, we found novel tissue-specific factors, including irx-1 and myrf-2, which control the integrity of the intestinal barrier and sarcomere structure during ageing respectively. This study demonstrates the complexity of ageing across worm tissues and highlights the power of tissue-specific transcriptomic profiling during ageing, which can serve as a resource to the field.

中文翻译：

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衰老诱导秀丽隐杆线虫的组织特异性转录组变化。

衰老是一个复杂的过程，具有跨组织的共同和独特特征。揭示个体组织中导致衰老的潜在过程对于破译有机体寿命的机制是必不可少的。Caenorhabditis elegans 是一种成熟的模式生物，通过发现控制其寿命的众多遗传途径，引领了衰老研究。然而，由于对组织病理学的描述有限以及在衰老过程中获得组织特异性分子变化，剖析蠕虫组织的衰老仍然具有挑战性。在这项研究中，我们从年轻和年老蠕虫的五个主要组织中分离出细胞，并分析了这些组织内由年龄引起的转录组变化。我们观察到不同组织的衰老差异显着，并在其中确定了不同的长寿调节因子。此外，我们发现了新的组织特异性因子，包括 irx-1 和 myrf-2，它们分别在衰老过程中控制肠道屏障和肌节结构的完整性。这项研究证明了蠕虫组织衰老的复杂性，并强调了衰老过程中组织特异性转录组分析的力量，这可以作为该领域的资源。