To explore useful natural compounds from indigenous medicinal plants, the cytotoxic properties from a methanolic extract of Boehmeria sieboldiana leaves against human cancer cell lines were isolated in the present study. After purification of the extract, seco-dehydroantofine B (1) together with two known phenanthroindolizine alkaloids, seco-dehydroantofine A (2) and septicine (3), were isolated. The structure of seco-dehydroantofine B was elucidated by performing comprehensive one- and two-dimensional nuclear magnetic resonance spectroscopy and high-resolution electrospray ionization mass spectrometry. The cytotoxicity of these compounds against five human tumor cell lines was evaluated. Compound 3 exhibited anti-tumor activity at IC₅₀ values of 50.0, 66.9, 50.0, and 153.7 µM against MKN1, SAS, HL-60, and THP-1 cells, respectively.

为了从本土药用植物中探索有用的天然化合物，在本研究中分离了Boehmeria sieboldiana叶子的甲醇提取物对人类癌细胞系的细胞毒性特性。提取物纯化后，分离出seco - dehydroantofine B ( 1 ) 以及两种已知的菲咯吲哚嗪生物碱，即seco - dehydroantofine A ( 2 ) 和 septicine ( 3 )。山高的结构-dehydroantofine B 通过进行综合一维和二维核磁共振波谱和高分辨率电喷雾电离质谱法进行了阐明。评估了这些化合物对五种人类肿瘤细胞系的细胞毒性。化合物3对 MKN1、SAS、HL-60 和 THP-1 细胞的 IC ₅₀值分别为 50.0、66.9、50.0 和 153.7 μM，表现出抗肿瘤活性。