We report a flexible and noninvasive method based on field-effect transistors hybridizing semiconducting single-walled carbon nanotubes for monitoring the effects of histamine on Ca²⁺ release from the intracellular stores of a nonexcitable cell. These nanodevices allowed us to evaluate the real-time electrophysiological activities of HeLa cells under the stimulation of histamine via the recording of the conductance changes of the devices. These changes resulted from the binding of histamine to its receptor type 1 on the HeLa cell membrane. Moreover, the effects of chlorphenamine, an antihistamine, on the electrophysiological activities of a single HeLa cell were also evaluated, indicating that the pretreatment of the cell with chlorpheniramine decreased intracellular Ca²⁺ release. Significantly, we only utilized a single nanodevice to perform the measurements for multiple cells pretreated with various concentrations of chlorphenamine. This enabled the statistically meaningful analysis of drug effects on cells without errors from device variations. Obtained results indicated the novel advantages of our method such as real-time monitoring and quantitative capability. Our devices, therefore, can be efficient tools for biomedical applications such as electrophysiology research and drug screening.

中文翻译：

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用于监测氯苯那敏对细胞内 Ca2+ 储存活性影响的半导体碳纳米管纳米器件

我们报告了一种基于场效应晶体管的灵活且非侵入性的方法，该方法与半导体单壁碳纳米管杂交，用于监测组胺对非兴奋细胞的细胞内储存的Ca ^{2+释放的影响。}这些纳米器件使我们能够通过记录器件的电导变化来评估 HeLa 细胞在组胺刺激下的实时电生理活动。这些变化是由于组胺与 HeLa 细胞膜上的 1 型受体结合所致。此外，还评估了抗组胺药扑尔敏对单个 HeLa 细胞电生理活性的影响，表明扑尔敏预处理细胞可降低细胞内 Ca ²⁺发布。值得注意的是，我们仅使用单个纳米装置对用不同浓度的氯苯那敏预处理的多个细胞进行测量。这使得能够对药物对细胞的影响进行统计学上有意义的分析，而不会因设备变化而产生错误。获得的结果表明了我们方法的新优势，例如实时监测和定量能力。因此，我们的设备可以成为生物医学应用的有效工具，例如电生理学研究和药物筛选。