Among glycolipids, mannosylerythritol lipids (MEL), are mild and environmentally friendly surfactants used in various industrial applications. MELs are produced by biofermentation using non-traditional oils with various water-soluble carbon sources as cell growth booster. This substrate affects the production yield and cost of MEL. In this research work, the non-traditional oils jatropha oil, karanja oil and neem oil were used as new substrates along with glucose, glycerol and honey as new water-soluble substrates. All these oils are new feedstocks for the production of MEL using Pseudozyma antarctica (ATCC 32657). Jatropha oil, karanja oil and neem oil with honey as substrates resulted in higher MEL yields of (8.07, 7.75, and 1.86) g/L and better cell growth of (8.07, 7.75, and 1.86) g/L, respectively, than non-traditional oils with glucose and glycerol as substrates. Neem oil gave a lower yield of MEL (1.54 g/L) as well as cell growth (6.06 g/L) compared to jatropha oil and karanja oil (7.03 and 6.17) g/L, respectively. Crude MEL from the fermentation broth was detected by thin-layer chromatography (TLC), Fourier transform infrared spectrommetry (FT-IR), high performance liquid chromatography (HPLC) and proton nuclear magnetic resonance spectroscopy ( 1 H NMR). Purified MEL has been used as an antimicrobial agent in cosmetic products associated with gram-positive and gram-negative bacteria and fungi.

中文翻译：

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具有水溶性底物的非传统油类作为细胞生长促进剂，用于通过 Pseudozyma antarctica (ATCC 32657) 生产具有抗菌活性的甘露糖基赤藓糖醇脂质

在糖脂中，甘露糖基赤藓糖醇脂 (MEL) 是一种温和且环保的表面活性剂，用于各种工业应用。MEL 是通过使用具有各种水溶性碳源的非传统油作为细胞生长促进剂的生物发酵生产的。该基板影响 MEL 的生产良率和成本。在这项研究工作中，以非传统油类麻风树油、卡兰贾油和印楝油为新底物，葡萄糖、甘油和蜂蜜为新的水溶性底物。所有这些油都是使用南极假酶 (ATCC 32657) 生产 MEL 的新原料。以蜂蜜为底物的麻风树油、卡兰贾油和印楝油分别导致更高的 MEL 产量（8.07、7.75 和 1.86）g/L 和更好的细胞生长（8.07、7.75 和 1.86）g/L，与以葡萄糖和甘油为底物的非传统油相比。与麻风树油和 karanja 油（7.03 和 6.17）g/L 相比，印楝油的 MEL 产量（1.54 g/L）和细胞生长（6.06 g/L）分别较低。通过薄层色谱法 (TLC)、傅里叶变换红外光谱法 (FT-IR)、高效液相色谱法 (HPLC) 和质子核磁共振光谱法 ( 1 H NMR) 检测来自发酵液的粗 MEL。纯化的 MEL 已被用作与革兰氏阳性和革兰氏阴性细菌和真菌相关的化妆品中的抗菌剂。通过薄层色谱法 (TLC)、傅里叶变换红外光谱法 (FT-IR)、高效液相色谱法 (HPLC) 和质子核磁共振光谱法 ( 1 H NMR) 检测来自发酵液的粗 MEL。纯化的 MEL 已被用作与革兰氏阳性和革兰氏阴性细菌和真菌相关的化妆品中的抗菌剂。通过薄层色谱法 (TLC)、傅里叶变换红外光谱法 (FT-IR)、高效液相色谱法 (HPLC) 和质子核磁共振光谱法 ( 1 H NMR) 检测来自发酵液的粗 MEL。纯化的 MEL 已被用作与革兰氏阳性和革兰氏阴性细菌和真菌相关的化妆品中的抗菌剂。