BACKGROUND Calcium phosphate silicate (Ca5(PO4)2SiO4 or CPS) is a promising bioceramic for bone grafting. Iron (Fe) is a trace element in the human body that has been reported to enhance the mechanical strength of CPS ceramics. However, the exact biofunctions of Fe, combined with another human trace element, viz. silicon (Si), in CPS and the optimal dose for Fe addition must be further investigated. METHODS In vitro: the morphology, structure and cell adhesion were observed by SEM; the ability to promote osteogenic differentiation and mineralization was explored by ALP and alizarin red staining; the expression of osteogenic-specific genes and proteins was detected by PCR, WB and immunofluorescence. In vivo: Further exploration of bone regeneration capacity by establishing a skull defect model. RESULTS In vitro, we observed increased content of adhesion-related proteins and osteogenic-related genes expression of Fe-CPS compared with CPS, as demonstrated by immunofluorescence and polymerase chain reaction experiments, respectively. In vivo micro-computed tomography images, histomorphology, and undecalcified bone slicing also showed improved osteogenic ability of Fe-CPS bioceramics. CONCLUSION With the addition of Fe2O3, the new bone formation rate of the Fe-CPS scaffold after 12 weeks increased from 9.42% to 43.76%. Moreover, both in vitro and in vivo experimental outcomes indicated that Fe addition improved the CPS bioceramics in terms of their osteogenic ability by promoting the expression of osteogenic-related genes. Fe-CPS bioceramics can be employed as a novel material for bone tissue engineering on account of their outstanding new bone formation ability. THE TRANSLATIONAL POTENTIAL OF THIS ARTICLE This study suggests that Fe-CPS bioceramics can be employed as a novel material for bone tissue engineering on account of their outstanding new bone formation ability,which provides promising therapeutic implants and strategies for the treatment of large segmental bone defects.

中文翻译：

---

硅卡诺生物陶瓷中掺杂铁的有利成骨活性：体外和体内研究。

背景技术磷酸钙硅酸盐(Ca5(PO4)2SiO4或CPS)是一种用于骨移植的有前途的生物陶瓷。铁 (Fe) 是人体中的一种微量元素，据报道它可以增强 CPS 陶瓷的机械强度。然而，铁的确切生物功能与另一种人体微量元素相结合，即。必须进一步研究 CPS 中的硅 (Si) 和添加 Fe 的最佳剂量。方法体外：扫描电镜观察细胞形态、结构和细胞粘附情况；ALP和茜素红染色探讨了促进成骨分化和矿化的能力；通过PCR、WB和免疫荧光检测成骨特异性基因和蛋白的表达。体内：通过建立颅骨缺损模型进一步探索骨再生能力。结果 在体外，我们观察到与 CPS 相比，Fe-CPS 的粘附相关蛋白含量和成骨相关基因表达增加，分别通过免疫荧光和聚合酶链反应实验证明。体内微型计算机断层扫描图像、组织形态学和未脱钙骨切片也显示了 Fe-CPS 生物陶瓷的成骨能力提高。结论添加Fe2O3后，Fe-CPS支架12周后新骨形成率由9.42%提高到43.76%。此外，体外和体内实验结果表明，添加铁通过促进成骨相关基因的表达，提高了 CPS 生物陶瓷的成骨能力。Fe-CPS生物陶瓷由于其突出的新骨形成能力，可用作骨组织工程的新型材料。本文的转化潜力 本研究表明，Fe-CPS 生物陶瓷具有出色的新骨形成能力，可作为一种新型骨组织工程材料，为大节段性骨缺损的治疗提供了有前景的治疗植入物和策略。 .