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AnfO controls fidelity of nitrogenase FeFe protein maturation by preventing misincorporation of FeV-cofactor
Molecular Microbiology ( IF 2.6 ) Pub Date : 2022-02-27 , DOI: 10.1111/mmi.14890 Ana Pérez-González 1 , Emilio Jimenez-Vicente 1 , Alvaro Salinero-Lanzarote 2 , Derek F Harris 3 , Lance C Seefeldt 3 , Dennis R Dean 1
Molecular Microbiology ( IF 2.6 ) Pub Date : 2022-02-27 , DOI: 10.1111/mmi.14890 Ana Pérez-González 1 , Emilio Jimenez-Vicente 1 , Alvaro Salinero-Lanzarote 2 , Derek F Harris 3 , Lance C Seefeldt 3 , Dennis R Dean 1
Affiliation
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Azotobacter vinelandii produces three genetically distinct, but structurally and mechanistically similar nitrogenase isozymes designated as Mo-dependent, V-dependent, or Fe-only based on the heterometal contained within their associated active site cofactors. These catalytic cofactors, which provide the site for N2 binding and reduction, are, respectively, designated as FeMo-cofactor, FeV-cofactor, and FeFe-cofactor. Fe-only nitrogenase is a poor catalyst for N2 fixation, when compared to the Mo-dependent and V-dependent nitrogenases and is only produced when neither Mo nor V is available. Under conditions favoring the production of Fe-only nitrogenase a gene product designated AnfO preserves the fidelity of Fe-only nitrogenase by preventing the misincorporation of FeV-cofactor, which results in the accumulation of a hybrid enzyme that cannot reduce N2. These results are interpreted to indicate that AnfO controls the fidelity of Fe-only nitrogenase maturation during the physiological transition from conditions that favor V-dependent nitrogenase utilization to Fe-only nitrogenase utilization to support diazotrophic growth.
中文翻译：
AnfO 通过防止 FeV 辅因子的错误掺入来控制固氮酶 FeFe 蛋白成熟的保真度
Azotobacter vinelandii产生三种遗传上不同但在结构和机制上相似的固氮酶同工酶，根据它们相关的活性位点辅因子中包含的杂金属，它们被指定为 Mo 依赖性、V 依赖性或仅 Fe。这些为N 2结合和还原提供位点的催化辅因子分别称为FeMo-辅因子、FeV-辅因子和FeFe-辅因子。仅 Fe 固氮酶是 N 2的不良催化剂与 Mo 和 V 依赖性固氮酶相比，固定作用仅在 Mo 和 V 均不可用时产生。在有利于产生仅Fe固氮酶的条件下，称为AnfO的基因产物通过防止FeV-辅因子的错误掺入来保持仅Fe固氮酶的保真度，这导致不能还原N 2的杂合酶的积累。这些结果被解释为表明在从有利于 V 依赖性固氮酶利用到仅利用铁固氮酶以支持固氮生长的条件的生理转变过程中，AnfO 控制了纯铁固氮酶成熟的保真度。
更新日期：2022-02-27
中文翻译：
AnfO 通过防止 FeV 辅因子的错误掺入来控制固氮酶 FeFe 蛋白成熟的保真度
Azotobacter vinelandii产生三种遗传上不同但在结构和机制上相似的固氮酶同工酶，根据它们相关的活性位点辅因子中包含的杂金属，它们被指定为 Mo 依赖性、V 依赖性或仅 Fe。这些为N 2结合和还原提供位点的催化辅因子分别称为FeMo-辅因子、FeV-辅因子和FeFe-辅因子。仅 Fe 固氮酶是 N 2的不良催化剂与 Mo 和 V 依赖性固氮酶相比，固定作用仅在 Mo 和 V 均不可用时产生。在有利于产生仅Fe固氮酶的条件下，称为AnfO的基因产物通过防止FeV-辅因子的错误掺入来保持仅Fe固氮酶的保真度，这导致不能还原N 2的杂合酶的积累。这些结果被解释为表明在从有利于 V 依赖性固氮酶利用到仅利用铁固氮酶以支持固氮生长的条件的生理转变过程中，AnfO 控制了纯铁固氮酶成熟的保真度。
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