Environmental enrichment effectively reduces anxiety-like indicators in animals, a potential co-treatment for diseases that generate variations in basal anxiety, such as alcoholism. Here we present an experimental design that allows investigating the effect of enrichment on anxiety-related behaviors using contextual aversive conditioning in zebrafish (Danio rerio). It was first observed whether animals conditioned with an aversive stimulus (electroshock paired with checked background tank) exhibited behavioral variations when previously held in barren (BE) or enriched (EE) environment. Enrichment reduced anxiety-like behaviors. Then, fish was exposed to four alcohol concentrations (0.00%, 0.10%, 0.50%, and 1.00%) after being held in BE or EE, and contextual fear conditioning was tested again. Fish showed dose-dependent and enrichment-related variation in anxiety-like behavior. Together, these results indicate that the experimental design in question is efficient in measuring behavior related to BE and EE, and that enrichment seems to impair the acquisition of dose-dependent effects of alcohol. Our results show that EE can alleviate deleterious effects caused by traumatic events, but it should also consider some effects of enrichment and alcohol exposure interaction.

环境丰富有效地减少了动物的焦虑样指标，这是一种潜在的共同治疗导致基础焦虑变化的疾病，如酗酒。在这里，我们提出了一个实验设计，该设计允许使用斑马鱼的情境厌恶条件来研究富集对焦虑相关行为的影响。）。首先观察到，当先前在贫瘠 (BE) 或富集 (EE) 环境中饲养时，受厌恶刺激（电击与检查的背景水箱配对）调节的动物是否表现出行为变化。富集减少了类似焦虑的行为。然后，将鱼置于 BE 或 EE 中后，暴露于四种酒精浓度（0.00%、0.10%、0.50% 和 1.00%），并再次测试情境恐惧条件反射。鱼在焦虑样行为中表现出剂量依赖性和浓缩相关的变化。总之，这些结果表明，所讨论的实验设计在测量与 BE 和 EE 相关的行为方面是有效的，并且富集似乎会损害酒精对剂量依赖性影响的获得。我们的研究结果表明，EE 可以减轻由创伤事件引起的有害影响，