As lowering crude protein (CP) in poultry diets continues to minimize amino acid excess, it is important to understand the limiting order of amino acids and the impact of their deficiencies. Therefore, a pair of experiments were conducted to observe the effects of individual amino acid deletions on growth performance, carcass traits, and nutrient utilization. Both experiments involved 3 control diets based on wheat and soybean meal, including a 210.0 g/kg CP industry control (IC), 186.7 g/kg CP positive control (PC) supplemented with feed-grade amino acids to match the IC amino acid profile, 186.7 g/kg CP negative control (NC) with reducing N corrected apparent metabolizable energy (AME_N) by 0.5 MJ/kg and removing feed-grade amino acids beyond L-Lys-HCl, DL-Met, and L-Thr from PC. Ten deletion diets where the following supplemented amino acids were individually removed from the PC: Val, Ile, Leu, Trp, Arg, His, Phe + Tyr, glycine equivalence (Gly_equi), Pro, and Energy (0.5 MJ/kg reduction in AME_N of the PC). All diets were formulated to contain similar concentrations of digestible Lys, total sulfur amino acid (TSAA) and Thr. Experimental diets were offered to broiler chickens from 15 to 22 d post–hatch in a cage study (Exp. 1) to gain digestibility and nutrient utilization data; whereas they were offered from 15 to 35 d post–hatch in a floor-pen study (Exp. 2) to gain performance and carcass yield data. The removal of supplemented Val, Arg, and Ile resulted in reduction on broiler performance (P < 0.05), and the removal of Val, Arg, Ile, and Gly_equi negatively influenced carcass traits (P < 0.05). Results from both experiments indicate that Val and Arg are co-limiting in wheat-soybean meal diets, but that Ile and Gly_equi may potentially limit breast and thigh development.

随着降低家禽日粮中的粗蛋白 (CP) 继续最大限度地减少氨基酸过量，了解氨基酸的限制顺序及其缺乏的影响非常重要。因此，进行了一对实验来观察单个氨基酸缺失对生长性能、胴体性状和养分利用的影响。两项实验均涉及 3 种基于小麦和豆粕的对照日粮，包括 210.0 g/kg CP 工业对照 (IC)、186.7 g/kg CP 阳性对照 (PC)，并添加饲料级氨基酸以匹配 IC 氨基酸谱, 186.7 g/kg CP 阴性对照 (NC) 与减少 N 校正表观代谢能 (AME _N) 0.5 MJ/kg 并从 PC 中去除 L-Lys-HCl、DL-Met 和 L-Thr 之外的饲料级氨基酸。十种缺失日粮，其中从 PC 中单独去除了以下补充氨基酸：Val、Ile、Leu、Trp、Arg、His、Phe + Tyr、甘氨酸当量 (Gly _equi )、Pro 和能量（减少 0.5 MJ/kg亚美尼亚_{_}电脑）。所有日粮的配方都含有相似浓度的可消化赖氨酸、总硫氨基酸（TSAA）和苏氨酸。在笼养研究（实验 1）中，在孵化后 15 至 22 天向肉鸡提供实验日粮，以获得消化率和养分利用数据；而在孵化后 15 至 35 天的地板围栏研究（实验 2）中提供了它们，以获得性能和胴体产量数据。去除添加的 Val、Arg 和 Ile 导致肉鸡生产性能下降（P  < 0.05），去除 Val、Arg、Ile 和 Gly _equi对胴体性状有负面影响（P  < 0.05）。两个实验的结果表明 Val 和 Arg 在小麦-豆粕日粮中是共同限制的，但 Ile 和 Gly _equi可能会限制乳房和大腿的发育。