Ceratocystis wilt and canker disease has devastated Acacia mangium plantations in south-east Asia. Current screening methodologies to identify resistant or tolerant germplasm use potted plants in a greenhouse as a preliminary screening to select material for field trials, but these tests are time-consuming, motivating a search for rapid screening protocols. In this study, inoculation procedures were tested on three species of Acacia, viz. A. mangium, A. crassicarpa and a hybrid of A. auriculiformis and A. mangium, using three isolates of Ceratocystis manginecans. Mycelial plugs were compared with spore suspensions as inoculum to infect artificial wounds on the stems of A. mangium potted plants. The rapid screening protocols involved inoculation of stem segments with mycelial plugs and detached phyllodes with a spore suspension, with susceptibility measured by lesion length on the stems or necrosis length on the phyllodes. Both mycelial plugs and spore suspensions produced a similar level of disease incidence, so either inoculum form can be used for an assay. The stem segments were prone to contamination by other fungi and to desiccation, while results from the potted plant and phyllode protocols showed similar trends of susceptibility among the Acacia clones and species. The ease, rapidity, and reproducibility of the phyllode inoculation protocol makes it a potential replacement for inoculation of potted plants as a preliminary screening protocol to identify disease tolerant A. mangium germplasm prior to field screening.

Ceratocystis 枯萎病和溃疡病已经摧毁了东南亚的金合欢种植园。目前鉴定抗性或耐受性种质的筛选方法使用温室中的盆栽植物作为初步筛选，以选择用于田间试验的材料，但这些测试非常耗时，促使人们寻求快速筛选方案。在这项研究中，接种程序在三种相思树上进行了测试，即。A. mangium , A. crassicarpa和A. auriculiformis和A. mangium的杂交种，使用三个分离的Ceratocystis manginecans. 将菌丝塞与孢子悬浮液作为接种物进行比较，以感染A. mangium盆栽植物茎上的人工伤口。快速筛选方案包括用菌丝塞接种茎段和用孢子悬浮液接种分离的叶状体，敏感性通过茎上的病变长度或叶状体上的坏死长度来测量。菌丝塞和孢子悬浮液都产生了相似的疾病发病率，因此任何一种接种形式都可以用于检测。茎段容易受到其他真菌的污染和干燥，而盆栽植物和叶状体方案的结果显示金合欢之间的易感趋势相似克隆和物种。叶状体接种方案的简便性、快速性和可重复性使其成为盆栽植物接种的潜在替代品，作为在田间筛选之前鉴定抗病A. mangium种质的初步筛选方案。