Lentinula edodes (Berk.) Pegler, the shiitake mushroom, is one of the most important mushrooms in the global mushroom industry. Although mycelium post ripeness and brown film (BF) formation are crucial for fruiting body initiation, the underlying molecular mechanisms of BF formation are largely unknown. In this study, proteomic quantification (relative and absolute) and metabolomic profiling of L. edodes were performed using isobaric tags and gas chromatography-mass spectroscopy, respectively. A total of 2,474 proteins were identified, which included 239 differentially expressed proteins. Notably, several proteins associated with autophagy were upregulated, including RPD3, TOR1, VAC8, VPS1, and VPS27. Transmission electron microscopy also indicated that autophagy occurred in post ripeness and BF formation. In time-dependent analysis of the metabolome, metabolites associated with oxidative stress and autophagy changed significantly, including mannitol, trehalose, myo-inositol, glucose, leucine, valine, glutamine, and 4-aminobutyric acid. Thus, oxidative stress and autophagy were important processes in post ripeness and BF formation in L. edodes, and new insights were gained into molecular mechanisms at proteome and metabolome levels.

香菇(Berk.) Pegler，香菇，是全球蘑菇产业中最重要的蘑菇之一。尽管菌丝体成熟后和棕色薄膜 (BF) 的形成对于子实体的启动至关重要，但 BF 形成的潜在分子机制在很大程度上是未知的。在这项研究中，蛋白质组学定量（相对和绝对）和代谢组学分析L. edodes分别使用等压标签和气相色谱-质谱法进行。共鉴定出 2474 种蛋白质，其中包括 239 种差异表达的蛋白质。值得注意的是，与自噬相关的几种蛋白质被上调，包括 RPD3、TOR1、VAC8、VPS1 和 VPS27。透射电子显微镜还表明自噬发生在成熟后和BF形成中。在代谢组的时间依赖性分析中，与氧化应激和自噬相关的代谢物发生了显着变化，包括甘露醇、海藻糖、肌醇、葡萄糖、亮氨酸、缬氨酸、谷氨酰胺和 4-氨基丁酸。因此，氧化应激和自噬是后成熟和BF形成的重要过程。L. edodes，并对蛋白质组和代谢组水平的分子机制获得了新的见解。