Dual adhesive unipolar polysaccharides synthesized by overlapping biosynthetic pathways in Agrobacterium tumefaciens,Molecular Microbiology

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Dual adhesive unipolar polysaccharides synthesized by overlapping biosynthetic pathways in Agrobacterium tumefaciens
Molecular Microbiology ( IF 2.6 ) Pub Date : 2022-02-21 , DOI: 10.1111/mmi.14887
Maureen C Onyeziri ₁ , Gail G Hardy ₁ , Ramya Natarajan ₁ , Jing Xu ₁ , Ian P Reynolds ₁ , Jinwoo Kim ₁ , Peter M Merritt ₁ , Thomas Danhorn ₁ , Michael E Hibbing ₁ , Alexandra J Weisberg ₂ , Jeff H Chang ₂ , Clay Fuqua ₁

Affiliation

Agrobacterium tumefaciens is a member of the Alphaproteobacteria that pathogenises plants and associates with biotic and abiotic surfaces via a single cellular pole. A. tumefaciens produces the unipolar polysaccharide (UPP) at the site of surface contact. UPP production is normally surface-contact inducible, but elevated levels of the second messenger cyclic diguanylate monophosphate (cdGMP) bypass this requirement. Multiple lines of evidence suggest that the UPP has a central polysaccharide component. Using an A. tumefaciens derivative with elevated cdGMP and mutationally disabled for other dispensable polysaccharides, a series of related genetic screens have identified a large number of genes involved in UPP biosynthesis, most of which are Wzx-Wzy-type polysaccharide biosynthetic components. Extensive analyses of UPP production in these mutants have revealed that the UPP is composed of two genetically, chemically, and spatially discrete forms of polysaccharide, and that each requires a specific Wzy-type polymerase. Other important biosynthetic, processing, and regulatory functions for UPP production are also revealed, some of which are common to both polysaccharides, and a subset of which are specific to each type. Many of the UPP genes identified are conserved among diverse rhizobia, whereas others are more lineage specific.

中文翻译：

根癌农杆菌重叠生物合成途径合成的双粘附单极性多糖

根癌农杆菌是 Alphaproteobacteria 的成员，它使植物致病并通过单个细胞极与生物和非生物表面结合。根癌农杆菌在表面接触部位产生单极性多糖（ UPP）。UPP 生产通常是表面接触诱导的，但第二信使环二鸟苷酸单磷酸 (cdGMP) 水平升高绕过了这一要求。多种证据表明 UPP 具有核心多糖成分。利用cdGMP升高且其他可有可无的多糖突变失效的根癌农杆菌衍生物，一系列相关的基因筛选已鉴定出大量参与UPP生物合成的基因，其中大部分是Wzx-Wzy型多糖生物合成成分。对这些突变体中 UPP 产生的广泛分析表明，UPP 由两种在遗传、化学和空间上离散的多糖形式组成，并且每种形式都需要特定的 Wzy 型聚合酶。还揭示了 UPP 生产的其他重要生物合成、加工和调节功能，其中一些功能是两种多糖所共有的，而其中一部分是每种类型所特有的。许多已鉴定的 UPP 基因在不同的根瘤菌中是保守的，而其他基因则更具谱系特异性。

更新日期：2022-02-21

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