Reagent red blood cells (RBCs) are prepared from donated whole blood, resulting in various combinations of blood group antigens. This inconsistency can be resolved by producing RBCs with uniform antigen expression. Induced pluripotent stem cells (iPSCs) generated directly from mature cells constitute an unlimited source for RBC production. We aimed to produce erythroid cells from iPSCs for diagnostic purposes. We hypothesized that cultured erythroid cells express surface antigens that can be recognized by blood group antibodies.

中文翻译：

---

用于诊断目的的与 OP9 细胞共培养的诱导多能干细胞的红系分化。

试剂红细胞 (RBC) 由捐赠的全血制备而成，可产生各种血型抗原组合。这种不一致可以通过产生具有统一抗原表达的红细胞来解决。直接从成熟细胞产生的诱导多能干细胞 (iPSC) 构成了 RBC 生产的无限来源。我们的目标是从 iPSC 中生产红细胞用于诊断目的。我们假设培养的红细胞表达可以被血型抗体识别的表面抗原。