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Polymorphism analysis of Valsa mali isolated from Xinjiang Province using ISSR markers
Agronomy Journal ( IF 2.0 ) Pub Date : 2022-02-17 , DOI: 10.1002/agj2.21000 Hongyan Liu 1 , Yanxia Wang 1 , Ling Tang 1 , Yalun Hao 1 , Huifang Deng 1 , Sifeng Zhao 2 , Chenzhong Jin 1 , Kaifa Guo 1
Agronomy Journal ( IF 2.0 ) Pub Date : 2022-02-17 , DOI: 10.1002/agj2.21000 Hongyan Liu 1 , Yanxia Wang 1 , Ling Tang 1 , Yalun Hao 1 , Huifang Deng 1 , Sifeng Zhao 2 , Chenzhong Jin 1 , Kaifa Guo 1
Affiliation
Apple quality and quantity is reduced by valsa canker (caused by Valsa mali Miyabe et Yamada) in Xinjiang, China. To develop treatment options, we need to understand its genetic diversity, which was assessed at the molecular level. Eight primers were selected for ISSR-PCR analysis of 44 strains of apple valsa canker in Xinjiang. The results showed that eight primers amplified a total of 80 clear and reproducible bands including 80 polymorphic loci; the polymorphic loci rate was 100%. Nei's gene diversity index and Shannon's information index were both higher than 0.2 and 0.4 in different geographical regions, which indicated that the genetic diversity of V. mali in Xinjiang was very abundant. The cluster analysis indicated that there was no significant correlation between the genetic relationships and their geographical originals.
更新日期:2022-02-17