This study aimed to investigate the correlation between microRNA (miR)-4429 and epidermal growth factor receptor (EGFR), the expression and clinical significance of miR-4429 in patients with non-small cell lung cancer (NSCLC), and the relationship between miR-4429 and EGFR mutation in NSCLC patients. Blood samples were collected from 122 NSCLC patients and 72 healthy volunteers. miR-4429 expression and EGFR mRNA expression were detected by real-time quantitative PCR. Correlation between miR-4429 and EGFR was evaluated by dual‑luciferase reporter assay and the Pearson correlation analysis. The ability of serum miR‑4429 to discriminate between NSCLC patients and healthy controls, and to discriminate between EGFR wild-type (EGFR-W) and EGFR mutant-type (EGFR-M) patients was assessed using receiver operating characteristic analysis. The relationship between miR-4429 and NSCLC patients' survival was identified by Kaplan-Meier survival curves and log-rank test. The prognostic value of miR-4429 in NSCLC patients was evaluated by Cox regression analysis. miR-4429 could directly bind to EGFR. Serum miR-4429, decreased in NSCLC patients, was negatively correlated with serum EGFR mRNA expression in NSCLC patients. Additionally, miR-4429 had a high diagnostic value for screening NSCLC patients from healthy controls, and was independently correlated with survival prognosis of NSCLC patients. Moreover, miR‑4429 was decreased in EGFR-M patients, which had a certain screening ability for EGFR‑M patients. Our findings indicate that miR-4429 is negatively correlated with EGFR in NSCLC, and may function as a diagnostic and prognostic biomarker for NSCLC patients. Additionally, miR-4429 is associated with EGFR mutation in NSCLC patients.

中文翻译：

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失调的循环 miR-4429 作为一种新型的非侵入性生物标志物，与非小细胞肺癌患者的 EGFR 突变相关。

本研究旨在探讨microRNA (miR)-4429与表皮生长因子受体(EGFR)的相关性，miR-4429在非小细胞肺癌(NSCLC)患者中的表达及临床意义，以及miR-4429与表皮生长因子受体(EGFR)的关系。 NSCLC患者中的-4429和EGFR突变。从 122 名 NSCLC 患者和 72 名健康志愿者中采集血样。实时定量PCR检测miR-4429表达和EGFR mRNA表达。通过双荧光素酶报告基因分析和 Pearson 相关分析评估 miR-4429 和 EGFR 之间的相关性。使用接受者操作特征分析评估血清 miR-4429 区分 NSCLC 患者和健康对照以及区分 EGF​​R 野生型 (EGFR-W) 和 EGFR 突变型 (EGFR-M) 患者的能力。通过Kaplan-Meier生存曲线和时序检验确定miR-4429与NSCLC患者生存之间的关系。通过 Cox 回归分析评估 miR-4429 在 NSCLC 患者中的预后价值。miR-4429 可以直接与 EGFR 结合。NSCLC 患者血清 miR-4429 降低，与 NSCLC 患者血清 EGFR mRNA 表达呈负相关。此外，miR-4429对于从健康对照中筛选NSCLC患者具有很高的诊断价值，并且与NSCLC患者的生存预后独立相关。此外，EGFR-M患者的miR-4429降低，对EGFR-M患者具有一定的筛查能力。我们的研究结果表明，miR-4429 与 NSCLC 中的 EGFR 呈负相关，并且可能作为 NSCLC 患者的诊断和预后生物标志物。此外，