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Oxidative stress and metal homeostasis alterations in Danio rerio (zebrafish) under single and combined carbamazepine, acetamiprid and cadmium exposures
Aquatic Toxicology ( IF 4.1 ) Pub Date : 2022-02-13 , DOI: 10.1016/j.aquatox.2022.106122
Gabriel de Farias Araujo 1 , Lorena Oliveira Souza Soares 2 , Sidney Fernandes Sales Junior 1 , Leandro Vargas Barreto de Carvalho 3 , Rafael Christian Chávez Rocha 4 , Tatiana Saint'Pierre 4 , Rachel Ann Hauser-Davis 5 , Fábio Veríssimo Correia 6 , Enrico Mendes Saggioro 5
Affiliation  

Contaminants of emerging concern (CEC) are routinely detected in aquatic environments, especially pharmaceuticals, such as carbamazepine (CBZ), and neonicotinoid pesticides, like acetamiprid (ACT). CECs can interact with each other and with other legislated contaminants like Cd, resulting in unknown effects. Most studies evaluate only the effects of single contaminant exposures on aquatic biota. Therefore, the aim of the present study was to assess the effects of both single and combined CBZ, ACT and Cd exposures on zebrafish brain and liver oxidative stress parameters and metal homeostasis. The biomarkers catalase (CAT), glutathione-S-transferase (GST), total thiols (TOT), metallothionein (MT) and malondialdehyde (MDA) and the essential elements Ca, Cu, K, Na, Mg, Mn and Zn were evaluated after 96-hour static exposures. CBZ, ACT and Cd single (brain and liver) and combined (liver) treatments resulted in oxidative effects in both fish organs, also leading to metal (Ca, Mg, K, Mn, Zn and Cu) homeostasis alterations. ACT exposure resulted in the greatest adverse effects in the brain, while CBZ was the cause of major element homeostasis and oxidative stress alterations in the liver. Lower LPO levels were observed in the combined treatments compared to single treatments, suggesting interactions and contaminant effect attenuation. This study is the first to evaluate the initial effects of combined CBZ, ACT and Cd exposures in zebrafish, paving the way for further investigations concerning other biomarkers during longer exposure times.



中文翻译:

单一和联合卡马西平、啶虫脒和镉暴露下斑马鱼(斑马鱼)的氧化应激和金属稳态变化

在水环境中经常检测到新出现的关注污染物 (CEC),特别是药物,如卡马西平 (CBZ) 和新烟碱类杀虫剂,如啶虫脒 (ACT)。CEC 可以相互影响,也可以与 Cd 等其他法定污染物相互作用,从而产生未知的影响。大多数研究仅评估单一污染物暴露对水生生物群的影响。因此,本研究的目的是评估单一和联合 CBZ、ACT 和 Cd 暴露对斑马鱼大脑和肝脏氧化应激参数和金属稳态的影响。评估了生物标志物过氧化氢酶 (CAT)、谷胱甘肽-S-转移酶 (GST)、总硫醇 (TOT)、金属硫蛋白 (MT) 和丙二醛 (MDA) 以及必需元素 Ca、Cu、K、Na、Mg、Mn 和 Zn经过 96 小时的静态暴露后。CBZ, ACT 和 Cd 单一(脑和肝)和联合(肝)处理导致两种鱼类器官的氧化作用,也导致金属(Ca、Mg、K、Mn、Zn 和 Cu)稳态改变。ACT 暴露对大脑造成最大的不利影响,而 CBZ 是肝脏中主要元素稳态和氧化应激改变的原因。与单一处理相比,在联合处理中观察到较低的 LPO 水平,表明相互作用和污染效应减弱。这项研究首次评估了斑马鱼联合 CBZ、ACT 和 Cd 暴露的初始影响,为在更长的暴露时间内进一步研究其他生物标志物铺平了道路。Zn 和 Cu) 稳态变化。ACT 暴露对大脑造成最大的不利影响,而 CBZ 是肝脏中主要元素稳态和氧化应激改变的原因。与单一处理相比,在联合处理中观察到较低的 LPO 水平,表明相互作用和污染效应减弱。这项研究首次评估了斑马鱼联合 CBZ、ACT 和 Cd 暴露的初始影响,为在更长的暴露时间内进一步研究其他生物标志物铺平了道路。Zn 和 Cu) 稳态变化。ACT 暴露对大脑造成最大的不利影响,而 CBZ 是肝脏中主要元素稳态和氧化应激改变的原因。与单一处理相比,在联合处理中观察到较低的 LPO 水平,表明相互作用和污染效应减弱。这项研究首次评估了斑马鱼联合 CBZ、ACT 和 Cd 暴露的初始影响,为在更长的暴露时间内进一步研究其他生物标志物铺平了道路。表明相互作用和污染效应衰减。这项研究首次评估了斑马鱼联合 CBZ、ACT 和 Cd 暴露的初始影响,为在更长的暴露时间内进一步研究其他生物标志物铺平了道路。表明相互作用和污染效应衰减。这项研究首次评估了斑马鱼联合 CBZ、ACT 和 Cd 暴露的初始影响,为在更长的暴露时间内进一步研究其他生物标志物铺平了道路。

更新日期:2022-02-16
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