In dentistry, indirect diagnostic methods such as electrical sensibility testing and pulse oximetry are used to assess the status of the pulp. Our study aimed to determine the correlation between hemoglobin oxygen saturation and vascular volume density (Vvasc). We also wanted to examine an electrical sensibility test and the volume density of myelinated nerve fibers (Vnerv). Twenty-six intact permanent premolars were included in the study. For histological analysis, the pulp tissue was stained with hematoxylin-eosin and immunohistochemically for von Willebrand factor and S100 to detect blood vessels and myelinated nerve fibers, respectively. The stereological analysis was used to determine the Vvasc and Vnerv. Statistical analysis was done using the Pearson correlation test and Welch's ANOVA test. Histological analysis showed that the pulp tissue was strongly vascularized and innervated. A significant positive correlation was found between Vvasc and hemoglobin oxygen saturation levels (p=0.030). A significant negative correlation was found between Vnerv and the lowest electrical voltage that patient felt (p=0.033). According to the maturity of the dental apex, teeth were divided into a group with open (N=6, OA group) and closed apex (N=20, CA group). We found that pulps in the CA group had higher Vnerv than the OA group (p=0.037). In contrast, there were no significant differences in Vvasc of the pulp tissue (p=0.059), oxygen saturation (p=0.907), or electrical voltage (p=0.113) between both groups. We can conclude that the measurement of pulse oximetry and electrical sensibility test reflect the morphology of healthy pulp tissue independently of the maturity of the dental apex.

中文翻译：

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牙科临床实践中使用的活力测试与牙髓组织学分析的比较。

在牙科中，使用电敏感性测试和脉搏血氧饱和度等间接诊断方法来评估牙髓的状态。我们的研究旨在确定血红蛋白氧饱和度与血管容积密度 (Vvasc) 之间的相关性。我们还想检查电敏感性测试和有髓神经纤维 (Vnerv) 的体积密度。研究中包括了 26 颗完整的永久性前磨牙。对于组织学分析，用苏木精-伊红对牙髓组织进行染色，并对血管性血友病因子和 S100 进行免疫组织化学染色，以分别检测血管和有髓神经纤维。立体学分析用于确定 Vvasc 和 Vnerv。使用 Pearson 相关检验和 Welch 的 ANOVA 检验进行统计分析。组织学分析表明，牙髓组织具有强烈的血管化和神经支配。在 Vvasc 和血红蛋白氧饱和度水平之间发现了显着的正相关性 (p=0.030)。在 Vnerv 和患者感受到的最低电压之间发现了显着的负相关 (p=0.033)。根据牙尖成熟度将牙齿分为开放组（N=6，OA组）和封闭组（N=20，CA组）。我们发现 CA 组的牙髓 Vnerv 高于 OA 组 (p=0.037)。相比之下，两组间牙髓组织的 Vvasc (p=0.059)、氧饱和度 (p=0.907) 或电压 (p=0.113) 没有显着差异。