The use of newly harvested corn in feed causes wet droppings in broilers and increased feed cost which was termed as “new season grain problem”. The present study was conducted to evaluate the proteomic profile of newly harvested corn and the subsequent influence on intestinal microbiol community for broiler chickens. Newly harvested corn stored for either half a month (HM) or two months (TM) was used, and the pasting properties, total soluble sugars, and proteomic analysis technology was used to explore the influence of storage on natural aging corn properties. Additionally, seventy-two 7-day-old Ross 308 male broiler chicken were fed with different stored corn. Apparent metabolizable energy (AME), digesta viscosity, intestinal morphology and microbiota were examined to explore the influence of feed corn storage on broiler chickens. Pasting properties in the TM corn exhibited decreased viscoelastic properties. Proteomic studies found a total of 26 proteins that were differentially expressed between the two treatment groups. Proteins involved in starch and polysaccharides biosynthesis were upregulated in TM compared with HM. Chickens fed on TM diet had higher relative energy utilization compared to the HM birds. With increased corn storage, the relative digesta viscosity decreased significantly (P ≤ 0.05). The total number of goblet cells and lymphocytes was lower in chickens fed the TM diet. The microbiota data showed that the TM chickens had decreased abundance of diarrheal bacteria such as Hungatella hathewayi and Bacteroides fragilis, and increased butyrate-producing bacteria such as Alistipes compared to the HM chickens. Storage of newly harvested corn induced the synthetic reaction of large molecules and changed the solubility of starch and protein with increasing soluble sugars and decreasing pasting properties that may improve the fermentation of intestinal microbiota, improve the energy utilization and protect gut health without the risk of diarrhea.

中文翻译：

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洞察新收获玉米的蛋白质组学特征和肉鸡肠道微生物群的宏基因组分析

在饲料中使用新收获的玉米会导致肉鸡出现湿便并增加饲料成本，这被称为“新季节谷物问题”。本研究旨在评估新收获玉米的蛋白质组学特征以及随后对肉鸡肠道微生物群落的影响。采用贮藏半个月（HM）或贮藏两个月（TM）的新收玉米，利用糊化特性、总可溶性糖和蛋白质组学分析技术探讨贮藏对自然老化玉米特性的影响。此外，72 只 7 日龄的 Ross 308 雄性肉鸡饲喂不同的储存玉米。检查表观代谢能 (AME)、食糜粘度、肠道形态和微生物群，以探索饲料玉米储存对肉鸡的影响。TM 玉米中的糊化特性表现出降低的粘弹性特性。蛋白质组学研究发现，两个治疗组之间共有 26 种蛋白质表达差异。与 HM 相比，TM 中参与淀粉和多糖生物合成的蛋白质上调。与 HM 鸡相比，以 TM 饮食喂养的鸡具有更高的相对能量利用率。随着玉米贮藏量的增加，食糜的相对粘度显着降低（P ≤ 0.05）。喂食 TM 饮食的鸡的杯状细胞和淋巴细胞总数较低。微生物群数据显示，与 HM 鸡相比，TM 鸡减少了腹泻细菌（如 Hungatella hathewayi 和脆弱拟杆菌）的丰度，并增加了产丁酸盐的细菌（如 Alistipes）。