The tomb KV 40 in the Kings' Valley (Luxor, Egypt) has revealed mummified and highly fragmented remains of 83 adults, children, new-borns and infants, some of whom were, according to inscriptions, 18th dynasty king's sons or daughters (∼1370 BCE). This tomb was then secondarily reused by priestly families during the 22nd to early 25th dynasty (∼900-700 BCE). Radiocarbon dating of such bone remains would allow the evaluation of the ratio of burials by period and the identification of those from the 18th dynasty. As the bones were buried within a shaft tomb under hot and dry climatic conditions and have been partially burnt by fires, the applicability of radiocarbon dating on collagen extracted from them was uncertain and highly challenging. Prior to collecting samples, the state of collagen preservation in the bones had to be evaluated to ensure the feasibility of radiocarbon dating.

Following the method of Lebon et al. (2016), three ATR-FTIR systems (benchtop and portables) were used to determine the effective and appropriate calibration of each instrument thanks to a set of reference bones. From this, we provide methodological recommendations to ensure the reliability of the quantifications obtained whatever the instrument used. As a demonstration of the efficiency of the proposed methodology, portable ATR-FTIR spectroscopy has been tested in situ in KV 40, and the N %wt contents thus evaluated have been compared to ATR-FTIR analyses in a laboratory (using a different spectrometer) and to elemental analyses. The results obtained on thirteen KV 40 individuals clearly show quite good consistency on ATR-FTIR analyses between both spectrometers, validating the relevance of this on-site analytical approach with each spectrometer properly calibrated and measurements performed according to a strict protocol. Regarding the estimation of the nitrogen content, all the KV 40 samples are radiocarbon datable despite challenging preservation conditions. Discrepancies have been highlighted between some of the ATR-FTIR results and elemental analyses, the latter systematically providing higher N %wt than the former. For these samples, the C %wt contents are also very high, showing that these bones are contaminated by organic materials. This underlines the efficacy of the ATR-FTIR analysis specifically for the quantification of bone collagen.

The developed analytical approach based on the ATR-FTIR method and its mobile instrumentation clearly operates effectively on site and under challenging conditions, which opens up new perspectives for the collection of bone remains prior to radiocarbon dating.

国王谷（埃及卢克索）的 KV 40 墓出土了 83 具成人、儿童、新生儿和婴儿的木乃伊和高度碎片化的遗骸，根据铭文，其中一些是第 18 王朝国王的儿女（~公元前 1370 年）。在第 22 至第 25 王朝初期（约公元前 900-700 年），该墓被祭司家族二次使用。对这些骨骼遗骸进行放射性碳测年可以评估不同时期的墓葬比例，并识别出 18 王朝的墓葬。由于这些骨头在炎热干燥的气候条件下被埋在竖井墓中，并且部分被大火烧毁，因此放射性碳年代测定法对从中提取的胶原蛋白的适用性不确定且极具挑战性。在采集样本之前，

遵循 Lebon 等人的方法。（2016 年），三个 ATR-FTIR 系统（台式和便携式）用于确定每个仪器的有效和适当校准，这要归功于一组参考骨骼。由此，我们提供方法建议以确保无论使用何种仪器所获得的量化结果的可靠性。作为所提出方法的效率的证明，便携式 ATR-FTIR 光谱已在原位进行了测试在 KV 40 中，因此评估的 N %wt 含量已与实验室中的 ATR-FTIR 分析（使用不同的光谱仪）和元素分析进行​​了比较。在 13 名 KV 40 个体上获得的结果清楚地表明，两个光谱仪之间的 ATR-FTIR 分析具有相当好的一致性，验证了这种现场分析方法与每个光谱仪正确校准和根据严格协议进行测量的相关性。关于氮含量的估计，尽管保存条件具有挑战性，但所有 KV 40 样品都是可放射性碳数据的。一些 ATR-FTIR 结果和元素分析之间存在差异，后者系统地提供了比前者更高的 N%wt。对于这些样品，C %wt 含量也非常高，表明这些骨头被有机物质污染。这强调了 ATR-FTIR 分析专门用于骨胶原定量的功效。

所开发的基于 ATR-FTIR 方法的分析方法及其移动仪器显然可以在现场和具有挑战性的条件下有效运行，这为在放射性碳测年之前收集骨骼遗骸开辟了新的前景。