Gendered burial practices that differentiate between men and women by the way the body was placed were used over large parts of Central Europe in the Late Neolithic and Early Bronze Age (c. 2900–1600 BC). Until recently, it was unknown if the sex-based differentiation of bodies was extended to children, and if the biological sex of the children matched the classification as men and women placed on the left/right side in the opposite orientation.

We applied nanoflow liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) to identify sex-specific peptides in human tooth enamel in 75 children under 12 years at death buried at one of the largest Early Bronze Age cemeteries in Europe, Franzhausen I, Austria, 70 of which produced reliable results.

The study confirmed that the sex of the children corresponds to the gendered body position in 98.4% of cases. For burials in which the gendered sidedness and orientation are not internally consistent with the male or female pattern, we found that the sidedness of the body corresponds to the sex of the children rather than the orientation.

在新石器时代晚期和青铜时代早期（约公元前 2900 年至 1600 年），中欧大部分地区采用了通过尸体放置方式区分男性和女性的性别葬礼。直到最近，还不知道基于性别的身体分化是否扩展到儿童，以及儿童的生理性别是否与男性和女性以相反方向放置在左侧/右侧的分类相匹配。

我们应用纳流液相色谱-串联质谱 (nanoLC-MS/MS) 来鉴定 75 名 12 岁以下儿童死亡时人类牙釉质中的性别特异性肽，这些儿童埋葬在欧洲最大的青铜时代早期墓地之一，Franzhausen I，奥地利，其中 70 个产生了可靠的结果。

该研究证实，在 98.4% 的病例中，儿童的性别与性别的身体位置相对应。对于性别侧向和方向与男性或女性模式内部不一致的墓葬，我们发现身体的侧向对应于儿童的性别而不是方向。