SxsA, a novel surface protein mediating cell aggregation and adhesive biofilm formation of Staphylococcus xylosus,Molecular Microbiology

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SxsA, a novel surface protein mediating cell aggregation and adhesive biofilm formation of Staphylococcus xylosus
Molecular Microbiology ( IF 2.6 ) Pub Date : 2022-01-24 , DOI: 10.1111/mmi.14884
Carolin J Schiffer ₁ , Christoph Schaudinn ₂ , Matthias A Ehrmann ₁ , Rudi F Vogel ₁

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Biofilm formation of staphylococci has been an emerging field of research for many years. However, the underlying molecular mechanisms are still not fully understood and vary widely between species and strains. The aim of this study was to identify new effectors impacting biofilm formation of two Staphylococcus xylosus strains. We identified a novel surface protein conferring cell aggregation, adherence to abiotic surfaces, and biofilm formation. The S. xylosus surface protein A (SxsA) is a large protein occurring in variable sizes. It lacks sequence similarity to other staphylococcal surface proteins but shows similar structural domain organization and functional features. Upon deletion of sxsA, adherence of S. xylosus strain TMW 2.1523 to abiotic surfaces was completely abolished and significantly reduced in TMW 2.1023. Macro- and microscopic aggregation assays further showed that TMW 2.1523 sxsA mutants exhibit reduced cell aggregation compared with the wildtype. Comparative genomic analysis revealed that sxsA is part of the core genome of S. xylosus, Staphylococcus paraxylosus, and Staphylococcus nepalensis and additionally encoded in a small group of Staphylococcus cohnii and Staphylococcus saprophyticus strains. This study provides insights into protein-mediated biofilm formation of S. xylosus and identifies a new cell wall-associated protein influencing cell aggregation and biofilm formation.

中文翻译：

SxsA，一种介导木糖葡萄球菌细胞聚集和粘附生物膜形成的新型表面蛋白

多年来，葡萄球菌的生物膜形成一直是一个新兴的研究领域。然而，潜在的分子机制仍未完全了解，并且物种和菌株之间差异很大。本研究的目的是确定影响两种木糖葡萄球菌菌株生物膜形成的新效应物。我们确定了一种新的表面蛋白，可赋予细胞聚集、粘附到非生物表面和生物膜形成。木糖链球菌表面蛋白 A (SxsA) 是一种大小不一的大蛋白。它与其他葡萄球菌表面蛋白缺乏序列相似性，但显示出相似的结构域组织和功能特征。在删除sxs A 后，S. xylosus的粘附在 TMW 2.1023 中，对非生物表面的 TMW 2.1523 菌株完全消除并显着减少。宏观和微观聚集测定进一步表明，与野生型相比，TMW 2.1523 sxsA突变体表现出细胞聚集减少。比较基因组分析表明，sxs A 是木糖葡萄球菌、副木糖葡萄球菌和尼泊尔葡萄球菌核心基因组的一部分，另外编码在一小群科氏葡萄球菌和腐生葡萄球菌菌株中。本研究提供了对蛋白质介导的木糖链球菌生物膜形成的见解并确定了一种影响细胞聚集和生物膜形成的新细胞壁相关蛋白。

更新日期：2022-01-24

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