The corn leafhopper Dalbulus maidis causes corn production losses mainly by acting as vector of pathogenic mollicutes and virus. Transgenic technologies that encode insecticidal proteins from Bacillus thuringiensis (Bt) are focused on the management of lepidopteran larvae in transgenic plants that traditionally express only one Bt allele. Adding another allele in transgenic plants is a recent and promising approach to managing resistant larvae, and it is important to know how the additional allele affects infestation and injury of non-target pests. This study evaluated the influence of maize hybrids with the genes cry1F, cry1A.105, and cry2Ab2 in homozygosis and hemizygosis compared with a conventional hybrid on the occurrence of D. maidis, transmitted diseases, and grain yield. Experiments were performed in two locations and two crop seasons in Brazil using three isogenic transgenic hybrids with three Bt proteins in homozygosis and hemizygosis and one conventional non-Bt hybrid. The numbers of D. maidis were recorded on plants across the vegetative stage, and disease symptoms were assessed at reproductive stage. Grain yield was recorded at the end of experiments. Homozygous and hemizygous hybrids did not differ for D. maidis incidence and transmitted diseases. Homozygous and hemizygous hybrids exhibited similar grain yields, and the transgenic Bt hybrids provided greater yields than the conventional hybrid in the first season in both locations. In conclusion, transgenic hybrids expressing three Bt proteins in homozygosis do not favor D. maidis infestation and diseases transmission compared to the hemizygous versions, and Bt zygosity has no negative effect on grain yield.

玉米叶蝉Dalbulus maidis主要通过作为病原软体动物和病毒的载体造成玉米产量损失。编码苏云金芽孢杆菌(Bt) 杀虫蛋白的转基因技术专注于管理传统上仅表达一个 Bt 等位基因的转基因植物中的鳞翅目幼虫。在转基因植物中添加另一个等位基因是管理抗性幼虫的最近且有希望的方法，了解额外的等位基因如何影响非目标害虫的侵扰和伤害非常重要。本研究评估了带有cry1F、cry1A.105和cry2Ab2基因的玉米杂种与常规杂种相比对纯合子和半合子的影响。D. maidis、传播疾病和粮食产量。在巴西的两个地点和两个作物季节进行了实验，使用了三种同基因转基因杂种，其中三种 Bt 蛋白处于纯合子和半合子中，另一种是常规的非 Bt 杂种。在整个营养阶段记录植物的D. maidis数量，并在生殖阶段评估疾病症状。在实验结束时记录谷物产量。D. maidis的纯合子和半合子杂种没有差异发病率和传播疾病。纯合子和半合子杂种表现出相似的谷物产量，转基因 Bt 杂种在两个地区的第一季比常规杂种提供更高的产量。总之，与半合子相比，表达三种纯合Bt蛋白的转基因杂种不利于D. maidis的侵染和疾病传播，并且Bt合子对谷物产量没有负面影响。