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The electron-bifurcating FeFe-hydrogenase Hnd is involved in ethanol metabolism in Desulfovibrio fructosovorans grown on pyruvate
Molecular Microbiology ( IF 2.6 ) Pub Date : 2022-01-23 , DOI: 10.1111/mmi.14881 Natalie Payne 1 , Arlette Kpebe 1 , Chloé Guendon 1 , Carole Baffert 1 , Julien Ros 1 , Régine Lebrun 2 , Yann Denis 3 , Laetitia Shintu 4 , Myriam Brugna 1
Molecular Microbiology ( IF 2.6 ) Pub Date : 2022-01-23 , DOI: 10.1111/mmi.14881 Natalie Payne 1 , Arlette Kpebe 1 , Chloé Guendon 1 , Carole Baffert 1 , Julien Ros 1 , Régine Lebrun 2 , Yann Denis 3 , Laetitia Shintu 4 , Myriam Brugna 1
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Desulfovibrio fructosovorans, a sulfate-reducing bacterium, possesses six gene clusters encoding six hydrogenases catalyzing the reversible oxidation of H2 into protons and electrons. Among them, Hnd is an electron-bifurcating hydrogenase, coupling the exergonic reduction of NAD+ to the endergonic reduction of a ferredoxin with electrons derived from H2. It was previously hypothesized that its biological function involves the production of NADPH necessary for biosynthetic purposes. However, it was subsequently demonstrated that Hnd is instead a NAD+-reducing enzyme, thus its specific function has yet to be established. To understand the physiological role of Hnd in D. fructosovorans, we compared the hnd deletion mutant with the wild-type strain grown on pyruvate. Growth, metabolite production and consumption, and gene expression were compared under three different growth conditions. Our results indicate that hnd is strongly regulated at the transcriptional level and that its deletion has a drastic effect on the expression of genes for two enzymes, an aldehyde ferredoxin oxidoreductase and an alcohol dehydrogenase. We demonstrated here that Hnd is involved in ethanol metabolism when bacteria grow fermentatively and proposed that Hnd might oxidize part of the H2 produced during fermentation generating both NADH and reduced ferredoxin for ethanol production via its electron bifurcation mechanism.
中文翻译：
电子分叉的 FeFe-氢化酶 Hnd 参与在丙酮酸上生长的 Desulfovibrio fructosovorans 的乙醇代谢
Desulfovibrio fructosovorans是一种硫酸盐还原菌，具有六个基因簇，编码六种氢化酶，催化 H 2可逆氧化成质子和电子。其中，Hnd是一种电子分叉氢化酶，将NAD +的放能还原与铁氧还蛋白的吸能还原与来自H 2的电子偶联。先前假设其生物学功能涉及生物合成目的所必需的NADPH的产生。然而，随后证明Hnd是一种NAD +还原酶，因此其具体功能尚未确定。了解 Hnd 在D. fructosovorans中的生理作用，我们将hnd缺失突变体与在丙酮酸上生长的野生型菌株进行了比较。比较了三种不同生长条件下的生长、代谢物生产和消耗以及基因表达。我们的结果表明hnd在转录水平上受到强烈调节，并且它的缺失对两种酶的基因表达有显着影响，醛铁氧还蛋白氧化还原酶和乙醇脱氢酶。我们在此证明，当细菌发酵生长时，Hnd 参与乙醇代谢，并提出 Hnd 可能会氧化发酵过程中产生的部分 H 2，通过其电子分叉机制产生 NADH 和还原铁氧还蛋白用于乙醇生产。
更新日期：2022-01-23
中文翻译：
电子分叉的 FeFe-氢化酶 Hnd 参与在丙酮酸上生长的 Desulfovibrio fructosovorans 的乙醇代谢
Desulfovibrio fructosovorans是一种硫酸盐还原菌，具有六个基因簇，编码六种氢化酶，催化 H 2可逆氧化成质子和电子。其中，Hnd是一种电子分叉氢化酶，将NAD +的放能还原与铁氧还蛋白的吸能还原与来自H 2的电子偶联。先前假设其生物学功能涉及生物合成目的所必需的NADPH的产生。然而，随后证明Hnd是一种NAD +还原酶，因此其具体功能尚未确定。了解 Hnd 在D. fructosovorans中的生理作用，我们将hnd缺失突变体与在丙酮酸上生长的野生型菌株进行了比较。比较了三种不同生长条件下的生长、代谢物生产和消耗以及基因表达。我们的结果表明hnd在转录水平上受到强烈调节，并且它的缺失对两种酶的基因表达有显着影响，醛铁氧还蛋白氧化还原酶和乙醇脱氢酶。我们在此证明，当细菌发酵生长时，Hnd 参与乙醇代谢，并提出 Hnd 可能会氧化发酵过程中产生的部分 H 2，通过其电子分叉机制产生 NADH 和还原铁氧还蛋白用于乙醇生产。
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