The development of trichomes, which protect plants against herbivores, is affected by various stresses. In tomato, previous studies showed that stress triggered JA signaling influences trichome formation, but the underlying mechanism is not fully resolved. Here, we found two C2H2 zinc finger proteins synergistically regulate JA-induced trichome formation in tomato. The naturally occurring mutations in H and its close homolog H-like gene in a spontaneous mutant, LA3172 cause severely affected trcihome development. Compared with respective single mutant, h/hl double mutant displayed more severe trichome defects in all tissues. Despite the partially redundant function, H and HL genes regulate the trichome formation in the spatially distinct manner, with HL more involved in hypocotyls and leaves, while H more involved in stems and sepals. Furthermore，the activity of H/HL is essential for JA-triggered trichome formation. JA signaling inhibitor SlJAZ2 represses the activity of H and HL via physical interaction, resulting in the activation of THM1, a negative regulator of trichome formation. Our results provide novel insight into the mechanism of the trichome formation in response to stress induced JA signaling in tomato.

中文翻译：

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H 和 HL 协同调节番茄中茉莉酸盐触发的毛状体形成。

保护植物免受食草动物侵害的毛状体的发育受到各种压力的影响。在番茄中，先前的研究表明，压力触发的 JA 信号会影响毛状体的形成，但其潜在机制尚未完全解决。在这里，我们发现两种 C2H2 锌指蛋白协同调节 JA 诱导的番茄毛状体形成。自发突变体 LA3172 中的 H 及其密切的同源 H 样基因中的自然发生突变会严重影响 trcihome 的发育。与各自的单突变体相比，h/hl双突变体在所有组织中表现出更严重的毛状体缺陷。尽管具有部分冗余功能，但 H 和 HL 基因以空间上不同的方式调节毛状体的形成，HL 更多地参与下胚轴和叶子，而 H 更多地参与茎和萼片。此外，H/HL 的活性对于 JA 触发的毛状体形成至关重要。JA 信号抑制剂 SlJAZ2 通过物理相互作用抑制 H 和 HL 的活性，导致 THM1 的激活，THM1 是毛状体形成的负调节因子。我们的研究结果为响应番茄中应激诱导的 JA 信号传导的毛状体形成机制提供了新的见解。