Grafting is an important horticultural technique used for many crop species. However, some scion/rootstock combinations are considered as incompatible due to poor graft union formation and subsequently high plant mortality. The early identification of graft incompatibility could allow the selection of non-viable plants before planting and would have a beneficial impact on research and development in the nursery sector. In general, visible phenotypes of grafted plants (size, root number, etc.) are poorly correlated with grafting success, but some studies have suggested that some polyphenols could be used as markers of graft incompatibility several months or years after grafting. However, much of the previous studies into metabolite markers of grafting success have not included all the controls necessary to unequivocally validate the markers proposed. In this study, we quantified 73 primary and secondary metabolites in nine hetero-grafts and six homo-grafted controls 33 days after grafting at the graft interface and in both the scion and rootstock woody tissues. Certain biomarker metabolites typical of a high stress status (such as proline, GABA and pallidol) were particularly accumulated at the graft interface of the incompatible scion/rootstock combination. We then used correlation analysis and generalized linear models to identify potential metabolite markers of grafting success measured one year after grafting. Here we present the first attempt to quantitatively predict graft compatibility and identify marker metabolites (especially asparagine, trans-resveratrol, trans-piceatannol and α-viniferin) 33 days after grafting, which was found to be particularly informative for homo-graft combinations.

中文翻译：

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识别葡萄树中成功嫁接联合形成的早期代谢标记。


嫁接是用于许多作物品种的重要园艺技术。然而，由于嫁接结合形成不良以及随后的高植物死亡率，一些接穗/砧木组合被认为是不相容的。早期识别嫁接不相容性可以在种植前选择无活力的植物，并对苗圃部门的研究和开发产生有益的影响。一般来说，嫁接植物的可见表型（大小、根数等）与嫁接成功的相关性较差，但一些研究表明，某些多酚可以作为嫁接后几个月或几年的嫁接不亲和性的标记。然而，之前对嫁接成功代谢标志物的许多研究并未包括明确验证所提出的标志物所需的所有对照。在这项研究中，我们在嫁接后 33 天对 9 个异种嫁接物和 6 个同种嫁接对照物中的嫁接界面以及接穗和砧木木质组织中的 73 种主要和次生代谢物进行了定量。某些典型的高胁迫状态生物标志物代谢物（如脯氨酸、GABA 和苍白球蛋白）尤其在不相容的接穗/砧木组合的嫁接界面处积累。然后，我们使用相关分析和广义线性模型来识别嫁接一年后测量的嫁接成功的潜在代谢标志物。在这里，我们首次尝试定量预测移植物相容性并鉴定移植后 33 天的标记代谢物（特别是天冬酰胺、反式白藜芦醇、反式白皮杉醇和 α-葡萄素），发现这对于同种移植物组合特别有用。