Crop rotation and biofertilizer application have historically been employed as efficient management strategies for soil-borne disease suppression through soil microbiome manipulation. However, how this occurs and to what extent the combination of methods affects the microbiota reconstruction of diseased soil is unknown. In this study, pineapple–banana rotation combined with biofertilizer application was used to suppress banana Fusarium wilt disease, and the effects on both bacterial and fungal communities were investigated using the MiSeq Illumina sequencing platform. Our results showed that pineapple–banana rotation significantly reduced Fusarium wilt disease incidence and the application of biofertilizer caused additional suppression. Bacterial and fungal communities thrived using rotation combined with biofertilizer application: taxonomic and phylogenetic α diversity of both bacteria and fungi increased along with disease suppression. Between the two strategies, biofertilizer application predominantly affected both bacterial and fungal community composition compared to rotation. Burkholderia genus may have been attributed to the general wilt suppression for its change in network structure and high relative importance in linear models. Our results indicated that pineapple–banana rotation combined with biofertilizer application has strong potential for the sustainable management of banana Fusarium wilt disease.

中文翻译：

---

菠萝-香蕉轮作结合生物肥施用重塑土壤微生物组诱导香蕉枯萎病抑制

作物轮作和生物肥料应用历来被用作通过土壤微生物组操作抑制土传疾病的有效管理策略。然而，这种情况是如何发生的，以及这些方法的组合在多大程度上影响了病害土壤的微生物群重建，目前尚不清楚。在这项研究中，菠萝-香蕉轮作结合生物肥料施用用于抑制香蕉枯萎病，并使用 MiSeq Illumina 测序平台研究对细菌和真菌群落的影响。我们的研究结果表明，菠萝-香蕉轮作显着降低了枯萎病的发病率，并且生物肥料的应用引起了额外的抑制。细菌和真菌群落通过轮作结合生物肥料应用茁壮成长： 细菌和真菌的α多样性随着疾病抑制而增加。在这两种策略之间，与轮作相比，生物肥料应用主要影响细菌和真菌群落组成。伯克霍尔德菌属可能由于其网络结构的变化和线性模型中的较高相对重要性而归因于一般的枯萎抑制。我们的研究结果表明，菠萝-香蕉轮作结合生物肥施用对香蕉枯萎病的可持续管理具有很大潜力。