Antifouling Electrochemical Biosensor Based on the Designed Functional Peptide and the Electrodeposited Conducting Polymer for CTC Analysis in Human Blood,Analytical Chemistry

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Antifouling Electrochemical Biosensor Based on the Designed Functional Peptide and the Electrodeposited Conducting Polymer for CTC Analysis in Human Blood
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-01-18 , DOI: 10.1021/acs.analchem.1c04787
Rui Han ₁ , Yang Li ₁ , Min Chen ₁ , Wanting Li ₁ , Caifeng Ding ₁ , Xiliang Luo ₁

Affiliation

Circulating tumor cells (CTCs) are considered reliable cancer biomarkers for the liquid biopsy of many types of tumors. The direct detection of CTCs in human blood with normal biosensors, however, remains challenging because of severe biofouling in blood that contains various proteins and a large number of cells. Herein, we report the construction of an antifouling electrochemical biosensor capable of assaying CTCs directly in blood, based on a designed multifunctional peptide and the electrodeposited conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT). The designed peptide possesses antifouling capability in complex biological media and specific recognition ability to capture breast cancer cells MCF-7. Meanwhile, electrodeposited PEDOT can promote electron transfer at the sensing interface, improve the signal-to-noise ratio for the detection, and thus enhance the sensitivity of the biosensor. The integration of the multifunctional peptide and conducting polymer PEDOT ensures that the developed biosensor is able to perform directly in blood samples without purification or separation. The antifouling electrochemical biosensor for the detection of MCF-7 cells exhibits a wide linear range over 4 orders, with a limit of detection (LOD) of 17 cells mL^–1. More interestingly, even when performing in 25% human blood, the biosensor still retains a linear response with an LOD of 22 cells mL^–1, without suffering significantly from biofouling in real blood. This work provides a promising strategy for the direct analysis of CTCs in human blood without a complicated pretreatment, and it may find practical application in the liquid biopsy of cancers.

中文翻译：

基于设计的功能肽和电沉积导电聚合物的防污电化学生物传感器用于人体血液中的 CTC 分析

循环肿瘤细胞 (CTC) 被认为是许多类型肿瘤液体活检的可靠癌症生物标志物。然而，由于血液中含有各种蛋白质和大量细胞的严重生物污染，用普通生物传感器直接检测人体血液中的 CTC 仍然具有挑战性。在此，我们报告了基于设计的多功能肽和电沉积导电聚合物聚 (3,4-乙烯二氧噻吩) (PEDOT) 构建的能够直接检测血液中 CTC 的防污电化学生物传感器。所设计的肽在复杂的生物介质中具有防污能力和捕获乳腺癌细胞 MCF-7 的特异性识别能力。同时，电沉积PEDOT可以促进传感界面的电子转移，提高检测的信噪比，从而提高生物传感器的灵敏度。多功能肽和导电聚合物 PEDOT 的集成确保了开发的生物传感器能够直接在血液样本中执行而无需纯化或分离。用于检测 MCF-7 细胞的防污电化学生物传感器表现出超过 4 个数量级的宽线性范围，检测限 (LOD) 为 17 个细胞 mL^–1。更有趣的是，即使在 25% 的人体血液中进行测试，生物传感器仍保持线性响应，LOD 为 22 个细胞 mL ^–1，而不会明显受到真实血液中的生物污染的影响。这项工作为直接分析人体血液中的 CTC 提供了一种有前景的策略，无需复杂的预处理，并且可能在癌症的液体活检中得到实际应用。

更新日期：2022-02-01

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