Conventional cuvette-based and microfluidics-based bacterial electroporation approaches have distinct advantages, but they are typically limited to relatively small sample volumes, reducing their utility for applications requiring high throughput such as the generation of mutant libraries. Here, we present a disposable, user-friendly microfluidic electroporation device capable of processing large volume bacterial samples yet requiring minimal device fabrication and straightforward operation. We demonstrate that the proposed device can outperform conventional cuvettes in a range of situations, including across Escherichia coli strains with a range of electroporation efficiencies, and we use its large-volume bacterial electroporation capability to generate a library of transposon mutants in the anaerobic gut commensal Bifidobacterium longum.

中文翻译：

---

M-TUBE：一种无需制造的微流控装置，用于需要最少组装的大体积细菌电穿孔

传统的基于比色皿和基于微流体的细菌电穿孔方法具有明显的优势，但它们通常仅限于相对较小的样本量，从而降低了它们在需要高通量的应用中的效用，例如突变库的生成。在这里，我们提出了一种一次性的、用户友好的微流体电穿孔装置，能够处理大量细菌样品，但需要最少的装置制造和简单的操作。我们证明，所提出的设备可以在一系列情况下优于传统比色皿，包括跨大肠杆菌菌株具有一系列电穿孔效率，我们利用其大容量细菌电穿孔能力在厌氧肠道共生长双歧杆菌中生成转座子突变体库。